Skip to main content
Log in

Analysis of the full-length VP2 protein of canine parvoviruses circulating in Hungary

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript


In recent years, the number of cases of disease caused by canine parvovirus 2 (CPV-2) in vaccinated dogs has increased. The aim of the present study was to identify CPV-2 strains present in Hungary. Forty-two out of 50 faecal specimens examined were positive, and 25 VP2 sequences were determined and analysed. Based on the current classification, the Hungarian viruses belong to New CPV-2a type, except two viruses that are recombinants of vaccine viruses and CPV-2a strains. The Tyr324Ile alteration was detected for the first time in Europe, and a “Hungarian-specific” substitution (Ala516Thr) was also identified in this study. The immunologically important parts of the currently spreading canine parvoviruses were examined and found to differ greatly from the vaccine strains that are widely used in Hungary.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.


  1. Buonavoglia C, Martella V, Pratelli A, Tempesta M, Cavalli A, Buonavoglia D, Bozzo G, Elia G, Decaro N, Carmichael L (2001) Evidence for evolution of canine parvovirus type 2 in Italy. J Gen Virol 82:3021–3025

    CAS  PubMed  Google Scholar 

  2. Demeter Z, Palade EA, Soós T, Farsang A, Jakab C, Rusvai M (2010) Misleading results of the MboII-based identification of type 2a canine parvovirus strains from Hungary reacting as type 2c strains. Virus Genes 41:37–42. doi:10.1007/s11262-010-0478-3

    Article  CAS  PubMed  Google Scholar 

  3. Hafenstein S, Bowman VD, Sun T, Nelson CD, Palermo LM, Chipman PR, Battisti AJ, Parrish CR, Rossmann MG (2009) Structural comparison of different antibodies interacting with parvovirus capsids. J Virol 83:5556–5566. doi:10.1128/JVI.02532-08

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  4. Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Ser 41:95–98

    CAS  Google Scholar 

  5. Hoelzer K, Shackelton LA, Parrish CR, Holmes EC (2008) Phylogenetic analysis reveals the emergence, evolution and dispersal of carnivore parvoviruses. J Gen Virol 89:2280–2289. doi:10.1099/vir.0.2008/002055-0

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  6. Hueffer K, Govindasamy L, Agbandje-McKenna M, Parrish CR (2003) Combinations of two capsid regions controlling canine host range determine canine transferrin receptor binding by canine and feline parvoviruses. J Virol 77:10099–10105. doi:10.1128/JVI.77.18.10099-10105.2003

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  7. López de Turiso JA, Cortés E, Ranz A, García J, Sanz A, Vela C, Casal JI (1991) Fine mapping of canine parvovirus B cell epitopes. J Gen Virol 72:2445–2456. doi:10.1099/0022-1317-72-10-2445

    Article  PubMed  Google Scholar 

  8. Kelly WR (1978) An enteric disease of dogs resembling feline panleukopenia virus. Australian Veterinary J 54:593

    Article  CAS  Google Scholar 

  9. Parrish CR, Aquadro CF, Strassheim ML, Evermann JF, Sgro JY, Mohammed HO (1991) Rapid antigenic-type replacement and DNA sequence evolution of canine parvovirus. J Virol 65:6544–6552

    CAS  PubMed Central  PubMed  Google Scholar 

  10. Parrish CR, O’Connell PH, Evermann JF, Carmichael LE (1985) Natural variation of canine parvovirus. Science 230:1046–1048. doi:10.1126/science.4059921

    Article  CAS  PubMed  Google Scholar 

  11. Reed AP, Jones EV, Miller TJ (1988) Nucleotide sequence and genome organization of canine parvovirus. J Virol 62:266–276

    CAS  PubMed Central  PubMed  Google Scholar 

  12. Zhang R, Yang S, Zhang W, Zhang T, Xie Z, Feng H, Wang S, Xia X (2010) Phylogenetic analysis of the VP2 gene of canine parvoviruses circulating in China. Virus Genes 40:397–402. doi:10.1007/s11262-010-0466-7

    Article  CAS  PubMed  Google Scholar 

Download references


This paper was supported by the TÁMOP 4.2.2.B-10/1-2010-0011 Grant of the Szent István University, Faculty of Veterinary Science.

Conflict of interest

None of the authors of this paper has a financial or personal relationship with other people or organisations that could inappropriately influence or bias the content of the paper.

Author information

Authors and Affiliations


Corresponding author

Correspondence to Attila Cságola.

Additional information

Nucleotide sequence data reported here are available in the GenBank database under the following Accession Numbers: KF539789-KF539805.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cságola, A., Varga, S., Lőrincz, M. et al. Analysis of the full-length VP2 protein of canine parvoviruses circulating in Hungary. Arch Virol 159, 2441–2444 (2014).

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: