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Location and architecture of an antibody-binding site of influenza A virus nucleoprotein

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Abstract

Amino acid positions recognized by monoclonal antibodies (MAbs) in the influenza A virus nucleoprotein (NP) have been reported. As these residues were scattered in the three-dimensional (3D) structure of NP, no patterns of the architecture of antibody-binding sites could be inferred. Here, we used site-specific mutagenesis and ELISA to screen the amino acids surrounding position 470 recognized by the MAb 3/1 as a linear epitope. Ten amino acid residues involved in the reaction of NP with the MAb 3/1 and the MAb 469/6 were identified. Our data are the first to outline a compact site recognized by MAbs in the 3D structure of the influenza virus NP.

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Acknowledgments

This study was supported by grant 13-04-00257 of the Russian Foundation for Basic Research. R.G.W. is supported by Contract No. HHSN266200700005C from the National Institute of Allergy and Infectious Disease, National Institutes of Health, Department of Health and Human Services, and by the American Lebanese Syrian Associated Charities (ALSAC).

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Authors and Affiliations

  1. D. I. Ivanovsky Institute of Virology, Gamaleya Str. 16, 123098, Moscow, Russia

    Natalia L. Varich, Galina K. Sadykova, Alexei G. Prilipov, Konstantin S. Kochergin-Nikitsky & Nikolai V. Kaverin

  2. Division of Virology, Department of Infectious Diseases, St. Jude Children’s Research Hospital, 262 Danny Thomas Place, Memphis, TN, 38105-3678, USA

    Robert G. Webster

  3. Department of Pathology, University of Tennessee, Memphis, TN, 38105, USA

    Robert G. Webster

Authors
  1. Natalia L. Varich
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  2. Galina K. Sadykova
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  3. Alexei G. Prilipov
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  4. Konstantin S. Kochergin-Nikitsky
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  5. Robert G. Webster
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  6. Nikolai V. Kaverin
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Correspondence to Natalia L. Varich.

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Varich, N.L., Sadykova, G.K., Prilipov, A.G. et al. Location and architecture of an antibody-binding site of influenza A virus nucleoprotein. Arch Virol 159, 1493–1497 (2014). https://doi.org/10.1007/s00705-013-1952-8

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  • DOI: https://doi.org/10.1007/s00705-013-1952-8

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