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Genetic dissection of a putative nucleolar localization signal in the coat protein of ourmia melon virus

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Abstract

Ourmiaviruses became the object of recent attention for their unusual taxonomic placements among plant viruses. The ourmia melon virus (OuMV) RNA3 encodes a 22-kDa coat protein (CP). Besides its role in virion formation, the OuMV CP facilitates systemic virus spread. In Nicotiana benthamiana, an eGFP-CP fusion protein was localized in the nucleus and preferentially in the nucleolus. By bioinformatics analysis, we identified an arginine- and lysine-rich region at the N-terminus of the CP. Here, we demonstrate by deletion and alanine scanning mutagenesis that this region in the CP is responsible for its preferential accumulation in the nucleolus of host cells.

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Acknowledgments

We wish to thank C. Perrone and R. Lenzi for their technical assistance in the laboratory and the greenhouse. Transgenic H2B-RFP Nicotiana benthamiana plants were a generous gift from M. M. Goodin.

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Authors and Affiliations

  1. Sez. di Torino, CNR, Istituto di Virologia Vegetale, Strada delle Cacce 73, 10135, Torino, Italy

    M. Rossi & M. Turina

  2. Dipartimento di Scienze della Vita e Biologia dei Sistemi, Università di Torino, Viale P.A. Mattioli 25, 10125, Torino, Italy

    A. Genre

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  1. M. Rossi
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Correspondence to M. Turina.

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Rossi, M., Genre, A. & Turina, M. Genetic dissection of a putative nucleolar localization signal in the coat protein of ourmia melon virus. Arch Virol 159, 1187–1192 (2014). https://doi.org/10.1007/s00705-013-1923-0

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  • DOI: https://doi.org/10.1007/s00705-013-1923-0

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