Abstract
The aG rabies virus strain has been attenuated through multiple passages in cells and is now used as a vaccine strain in China. We attempted to develop a reverse genetics system using the aG strain. Recombinant full-length genomic cDNA was flanked by a hammerhead ribozyme and the hepatitis delta virus ribozyme. Three helper plasmids encoding the nucleoprotein, the phosphoprotein, and the large protein were produced and introduced together with a plasmid containing the full-length aG viral genome into BHK-21 cells by transfection. Recombinant virus was successfully recovered from the cloned cDNA under the control of a CMV promoter driven by RNA polymerase II. The recombinant virus was confirmed by RT-PCR, and the titer of the recombinant virus was 6.2 log LD50.
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Wenqiang, J., Yin, X., Lan, X. et al. Development of a reverse genetics system for the aG strain of rabies virus in China. Arch Virol 159, 1033–1038 (2014). https://doi.org/10.1007/s00705-013-1919-9
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DOI: https://doi.org/10.1007/s00705-013-1919-9