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Archives of Virology

, Volume 159, Issue 5, pp 1181–1185 | Cite as

Complete genome sequence of invertebrate iridovirus IIV-25 isolated from a blackfly larva

  • Benoît Piégu
  • Sébastien Guizard
  • Tatsinda Spears
  • Corinne Cruaud
  • Arnault Couloux
  • Dennis K. Bideshi
  • Brian A. Federici
  • Yves BigotEmail author
Brief Report

Abstract

Members of the family Iridoviridae are animal viruses that infect only invertebrates and poikilothermic vertebrates. Invertebrate iridovirus 25 (IIV-25) was originally isolated from the larva of a blackfly (Simulium spp., order Diptera) found in the Ystwyth river near Aberystwyth, Wales. IIV-25 virions are icosahedral, have a diameter of ~130 nm, and contain a dsDNA genome of 204.8 kbp, with a G+C content of 30.32 %, that codes for 177 proteins. Here, we describe the complete genome sequence of this virus and its annotation. This is the fifth genome sequence of an invertebrate iridovirus reported.

Keywords

Iridoviridae Iridovirus dsDNA virus Insect Invertebrate MITE Intein 

Abbreviations

CDS

Coding DNA sequence

dsDNA

Double-stranded DNA

EMBL

European Molecular Biology Laboratory

ICTV

International Committee on Taxonomy of Viruses

IIV

Invertebrate iridovirus

Kbp

Kilobase pairs

MCP

Major capsid protein

MITE

Miniature transposable element

NLCDV

Nucleocytoplasmic large DNA virus

ORF

Open reading frame

Notes

Acknowledgments

This work was supported by the C.N.R.S. and Genoscope and funded by the Ministère de l’Education Nationale, de la Recherche et de la Technologie and the Groupement de Recherche CNRS 3546.

Supplementary material

705_2013_1918_MOESM1_ESM.docx (91 kb)
Supplementary material 1 (DOCX 90 kb)

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Copyright information

© Springer-Verlag Wien 2013

Authors and Affiliations

  • Benoît Piégu
    • 1
  • Sébastien Guizard
    • 1
  • Tatsinda Spears
    • 2
    • 3
  • Corinne Cruaud
    • 4
  • Arnault Couloux
    • 4
  • Dennis K. Bideshi
    • 2
    • 5
  • Brian A. Federici
    • 2
    • 3
  • Yves Bigot
    • 1
    Email author
  1. 1.UMR INRA-CNRS 7247, PRC, Centre INRA de NouzillyNouzillyFrance
  2. 2.Department of EntomologyUniversity of CaliforniaRiversideUSA
  3. 3.Interdepartmental Graduate Programs in Cell, Molecular and Developmental BiologyUniversity of CaliforniaRiversideUSA
  4. 4.CEA/Institut de Génomique GENOSCOPEEvry CedexFrance
  5. 5.Department of Natural and Mathematical SciencesCalifornia Baptist UniversityRiversideUSA

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