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Complete genome sequence of a novel endornavirus in the wheat sharp eyespot pathogen Rhizoctonia cerealis

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Abstract

We report here the presence of a novel double-stranded RNA (dsRNA) virus in an isolate (R0959) of the fungus Rhizoctonia cerealis, the causal agent of sharp eyespot of wheat in China. Sequence analysis showed that the dsRNA segment is 17,486 bp long and contains a single open reading frame (ORF) with the potential to encode a protein of 5,747 amino acids. The predicted protein contains conserved motifs of putative viral methyltransferase, helicase 1, and RNA-dependent RNA polymerase. Sequence similarity and phylogenetic analysis clearly place it in a distinct species within the genus Endornavirus, family Endornaviridae, and therefore we propose its name to Rhizoctonia cerealis endornavirus 1 (RcEV1). This is the first report of the full-length genomic sequence of a dsRNA mycovirus in R. cerealis.

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References

  1. Bharathan N, Saso H, Gudipati L, Bharathan S, Whited K, Anthony K (2005) Double-stranded RNA distribution and analysis among isolates of Rhizoctonia solani AG-2 to -13. Plant Path 54:196–203

    Article  CAS  Google Scholar 

  2. Chen Y, Li W, Zhang XX, Zhang BQ, Yu HS, Chen HG (2009) Composition and virulence of pathogen of wheat sharp eyespot in north latitude 33 of China (in Chinese). J Triticeae Crops 29:1110–1114

    Google Scholar 

  3. Darissa O, Willingmann P, Adam G (2010) Optimized approaches for the sequence determination of double-stranded RNA templates. J Virol Methods 169:397–403

    Article  CAS  PubMed  Google Scholar 

  4. Espach Y, Maree HJ, Burger JT (2012) Complete genome of a novel endornavirus assembled from next-generation sequence data. J Virol 86:13142

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  5. González García V, Portal Onco MA, Rubio Susan V (2006) Review: Biology and systematics of the form genus Rhizoctonia. Span J Agric Res 4:55–79

    Google Scholar 

  6. Hacker CV, Brasier CM, Buck KW (2005) A double-stranded RNA from a Phytophthora species is related to the plant endornaviruses and contains a putative UDP glycosyltransferase gene. J Gen Virol 86:1561–1570

    Article  CAS  PubMed  Google Scholar 

  7. Jian J, Lakshman DK, Tavantzis SM (1997) Association of distinct double-stranded RNAs with enhanced or diminished virulence in Rhizoctonia solani infecting potato. Mol Plant-Microbe Interact 10:1002–1009

    Article  CAS  Google Scholar 

  8. Liu H, Fu Y, Jiang D, Li G, Xie J, Peng Y, Yi X, Ghabrial SA (2009) A novel mycovirus that is related to the human pathogen Hepatitis E virus and rubi-like viruses. J Virol 83:1981–1991

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  9. Morris TJ, Dodds JA (1979) Isolation and analysis of double stranded RNA from virus-infected plant and fungal tissue. Phytopathology 69:854–858

    Article  CAS  Google Scholar 

  10. Moriyama H, Horiuchi H, Ntta T, Fukuhara T (1999) Unusual inheritance of evolutionarily-related double-stranded RNAs in interspecific hybrid between rice plants Oryza sativa and Oryza rufipogon. Plant Mol Biol 39:1127–1136

    Article  CAS  PubMed  Google Scholar 

  11. Nuss DL (2005) Hypovirulence: mycoviruses at the fungal–plant interface. Nat Rev Microbiol 3:632–642

    Article  CAS  PubMed  Google Scholar 

  12. Okada R, Kiyota E, Sabanadzovic S, Moriyama H, Fukuhara T, Saha P, Roossinck MJ, Severin A, Valverde RA (2011) Bell pepper endornavirus: molecular and biological properties, and occurrence in the genus Capsicum. J Gen Virol 92:2664–2673

    Article  CAS  PubMed  Google Scholar 

  13. Okada R, Yong CK, Valverde RA, Sabanadzovic S, Aoki N, Hotate S, Kiyota E, Moriyama H, Fukuhara T (2013) Molecular characterization of two evolutionarily distinct endornaviruses co-infecting common bean (Phaseolus vulgaris). J Gen Virol 94:220–229

    Article  CAS  PubMed  Google Scholar 

  14. Osaki H, Nakamura H, Sasaki A, Matsumoto N, Yoshida K (2006) An endornavirus from a hypovirulent strain of the violet root rot fungus, Helicobasidium mompa. Virus Res 118:143–149

    Article  CAS  PubMed  Google Scholar 

  15. Pfeiffer P (1998) Nucleotide sequence, genetic organization and expression strategy of the double-stranded RNA associated with the ‘447’ cytoplasmic male sterility trait in Vicia faba. J Gen Virol 79:2349–2358

    CAS  PubMed  Google Scholar 

  16. Roossinck MJ, Sabanadzovic S, Okada R, Valverde RA (2011) The remarkable evolutionary history of endornaviruses. J Gen Virol 92:2674–2678

    Article  CAS  PubMed  Google Scholar 

  17. Stielow B, Klenk HP, Menzel W (2011) Complete genome sequence of the first endornavirus from the ascocarp of the ectomycorrhizal fungus Tuber aestivum Vittad. Arch Virol 156:343–345

    Article  CAS  PubMed  Google Scholar 

  18. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony method. Mol Biol Evol 28:2731–2739

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  19. Tuomivirta TT, Kaitera J, Hantula J (2009) A novel putative virus of Gremmeniella abietina type B (Ascomycota: Helotiaceae) has a composite genome with endornavirus affinities. J Gen Virol 90:2299–2305

    Article  CAS  PubMed  Google Scholar 

  20. Villanueva F, Sabanadzovic S, Valverde RA, Navas-Castillo J (2012) Complete genome sequence of a double-stranded RNA virus from avocado. J Virol 86:1282–1283

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  21. Zheng L, Liu H, Zhang M, Cao X, Zhou E (2013) The complete genomic sequence of a novel mycovirus from Rhizoctonia solani AG-1 IA strain B275. Arch Virol 158:1609–1612

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

This work was supported by Jiangsu Agriculture Science and Technology Innovation Fund, CX(11)4015, National Science Foundation of China (30900928), and the fund earmarked for the China Agricultural Research System (CARS-3-1-17).

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The authors declare no conflict of interest.

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Correspondence to Kerong Wang.

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W. Li, T. Zhang contributed equally to this work.

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Li, W., Zhang, T., Sun, H. et al. Complete genome sequence of a novel endornavirus in the wheat sharp eyespot pathogen Rhizoctonia cerealis . Arch Virol 159, 1213–1216 (2014). https://doi.org/10.1007/s00705-013-1893-2

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  • DOI: https://doi.org/10.1007/s00705-013-1893-2

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