Abstract
To enable the differential PCR detection of Andean potato latent virus (APLV) and Andean potato mild mosaic virus (APMMV) strains, sense primers were designed that correspond to regions directly upstream of the coat protein genes. Their differentiating power was increased by A->C or T->C replacements in their 3’-terminal parts. Together with the broad-specificity antisense primer EM3, primer AL-a-mod3C detected all APLV strains tested, but none of the APMMV strains. Primer AM-a-mod4C yielded PCR products with all APMMV preparations, but also with some APLV preparations. Sequence analysis revealed that this was not due to a lack of primer specificity, but to the sensitive detection of contaminating APMMV in some of our APLV preparations.
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References
Gibbs AJ, Hecht-Poinar E, McKee RK (1966) Some properties of three related viruses: Andean potato latent, dulcamara mottle and ononis yellow mosaic. J Gen Microbiol 44:177–193
Fribourg CE, Jones RAC, Koenig R (1977) Host plant reactions, physical properties and serology of three isolates of Andean potato latent virus from Peru. Ann Appl Biol 86:373–380
Koenig R, Fribourg CE, Jones RAC (1979) Symptomatological, serological and electrophoretic diversity of isolates of Andean potato latent virus from different regions of the Andes. Phytopathology 69:748–752
Jones RAC, Fribourg CE (1978) Symptoms induced by Andean potato latent virus in wild and cultivated potatoes. Potato Res 21:121–127
Kreuze J, Koenig R, De Souza J, Vetten HJ, Muller G, Flores B, Ziebel H, Cuellar W (2013) The complete genome sequences of a Peruvian and a Colombian isolate of Andean potato latent virus and partial sequences of further isolates suggest the existence of two distinct potato-infecting tymovirus species. Virus Res 173:431–435
Dreher TW, Edwards MC, Gibbs AJ, Haenni A-L, Hammond RW, Jupin I, Koenig R, Sabanadzovic S, artelli GP (2012) Tymovirus In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds), Virus Taxonomy, Ninth Report of the ICTV. Elsevier/Academic Press, London, pp 946–952
Ding SW, Howe J, Keese P, Mackenzie A, Meek D, Osorio-Keese M, Skotnicki M, Srifah P, Torronen M, Gibbs A (1990) The tymobox, a sequence shared by most tymoviruses: its use in molecular studies of tymoviruses. Nucl Acids Res 18:1181–1187
Kwok S, Kellogg DE, McKinney N, Spasic D, Goda L, Levenson C, Sninsky J (1990) Effect of primer-template mismatches on the polymerase chain reaction: human immunodeficiency virus type 1 mode. Nucleic Acids Res 18:999–1005
Matsunaga J, Tomita Y, Tagami H (1995) Detection of point mutations in human tyrosinase gene by improved allele-specific amplification. Exp Dermatol 4:377–381
Newton CR, Graham A, Heptinstall LE, Powell SJ, Summers C, Kalsheker N, Smith JC, Markham AF (1989) Analysis of any point mutation in DNA. The amplification refractory mutation system (ARMS). Nucleic Acids Res 17:503–2516
Koenig R, Büttner G (2004) Strategies for the detection of potential Beet necrotic yellow vein virus genome recombinations which might arise as a result of growing A type coat protein gene-expressing sugar beets in soil containing B type virus. Trans Res 13:21–28
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We are greatly indebted to Mrs. Petra Lüddecke for excellent technical assistance.
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Koenig, R., Ziebell, H. Sequence-modified primers for the differential RT-PCR detection of Andean potato latent and Andean potato mild mosaic viruses in quarantine tests. Arch Virol 159, 1169–1173 (2014). https://doi.org/10.1007/s00705-013-1859-4
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DOI: https://doi.org/10.1007/s00705-013-1859-4