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Inhibitory effects of curcumin on dengue virus type 2-infected cells in vitro

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Abstract

Curcumin, a traditional Chinese and Indian treatment for many diseases, has recently been found to alter the in vitro infection processes of various viruses, including hepatitis C virus, human immunodeficiency virus, coxsackievirus, and Japanese encephalitis virus. The present study evaluated the cellular effects of curcumin in an in vitro (cellular) infection model of dengue virus. Within a dose range of 10 to 30 μM and a treatment period of 24 hours, the cytotoxicity of curcumin was low, as determined by MTT assays. Cells infected with dengue virus type 2 at a multiplicity of infection of 5 were treated with various concentrations of curcumin or the proteasome inhibitor MG132. Plaque assays, immunofluorescence analysis, western blots, and in-cell western assays were then performed. Treatment with 10, 15, and 20 μM curcumin decreased the number of plaques produced, caused an intracellular accumulation of viral proteins, and increased the level of Lys48 ubiquitin-conjugated proteins. At 20 μM curcumin, changes in cell and nuclear morphology and alterations in the actin cytoskeleton were also observed. Treatment with MG132 also reduced plaque production. These results show that curcumin can interfere with the infection processes of dengue virus and that this interference may not occur through direct effects on viral particle production but may result from curcumin’s effects on various cellular systems such as the cytoskeleton, the ubiquitin-proteasome system, or the apoptosis process.

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Acknowledgments

The authors would like to thank at University of Quindío, Gymol Group. This work was supported by Colciencias (departamento administrativo de ciencia tecnología e innovación) grant No. 1113-493-26231.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. GYMOL Group, Universidad del Quindío, Facultad de ciencias de la salud, Cll 12N, Armenia, Quindío, Colombia

    Leonardo Padilla-S, Andrés Rodríguez, María M. Gonzales & Jhon C. Castaño-O

  2. Medicina molecular y de translación Group, Universidad de Antioquia, Medellín, Colombia

    Juan C. Gallego-G

Authors
  1. Leonardo Padilla-S
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  2. Andrés Rodríguez
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  3. María M. Gonzales
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  4. Juan C. Gallego-G
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  5. Jhon C. Castaño-O
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Correspondence to Leonardo Padilla-S.

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Padilla-S, L., Rodríguez, A., Gonzales, M.M. et al. Inhibitory effects of curcumin on dengue virus type 2-infected cells in vitro . Arch Virol 159, 573–579 (2014). https://doi.org/10.1007/s00705-013-1849-6

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  • DOI: https://doi.org/10.1007/s00705-013-1849-6

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