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Crimean-Congo hemorrhagic fever virus nucleoprotein suppresses IFN-beta-promoter-mediated gene expression

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Abstract

Crimean-Congo hemorrhagic fever virus (CCHFV) is a member of the family Bunyaviridae and is a causative agent of severe hemorrhagic disease. Knowledge regarding the pathogenesis of CCHFV is limited due to the requirement for high-containment laboratories and the lack of an immunocompetent animal host. Previous studies have shown that CCHFV delays the activation of the human innate immune response, specifically, the type I interferon response. Our study results show that antagonism of the interferon-beta promoter is mediated by the nucleoprotein of CCHFV strain Hoti, while strains IbAr10200 and AP92 do not suppress the activity of the IFN-beta promoter. Our results also suggest that several viral factors may provide antagonistic action against the type I interferon response.

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References

  1. Elliott RM, Weber F (2009) Bunyaviruses and the type I interferon system. Viruses 1(3):1003–1021. doi:10.3390/v1031003

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  2. Akinci E, Bodur H, Leblebicioglu H (2013) Pathogenesis of Crimean-Congo hemorrhagic fever. Vector Borne Zoonotic Dis. doi:10.1089/vbz.2012.1061

    PubMed  Google Scholar 

  3. Zivcec M, Safronetz D, Scott D, Robertson S, Ebihara H, Feldmann H (2013) Lethal Crimean-Congo hemorrhagic fever virus infection in interferon alpha/beta receptor knockout mice is associated with high viral loads, proinflammatory responses, and coagulopathy. J Infect Dis. doi:10.1093/infdis/jit061

    Google Scholar 

  4. Bereczky S, Lindegren G, Karlberg H, Akerstrom S, Klingstrom J, Mirazimi A (2010) Crimean-Congo hemorrhagic fever virus infection is lethal for adult type I interferon receptor-knockout mice. J Gen Virol 91(Pt 6):1473–1477

    Article  CAS  PubMed  Google Scholar 

  5. Bente DA, Alimonti JB, Shieh WJ, Camus G, Stroher U, Zaki S, Jones SM (2010) Pathogenesis and immune response of Crimean-Congo hemorrhagic fever virus in a STAT-1 knockout mouse model. J Virol 84(21):11089–11100

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  6. Bowick GC, Airo AM, Bente DA (2012) Expression of interferon-induced antiviral genes is delayed in a STAT1 knockout mouse model of Crimean-Congo hemorrhagic fever. Virol J 9:122. doi:10.1186/1743-422X-9-122

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  7. Andersson I, Karlberg H, Mousavi-Jazi M, Martinez-Sobrido L, Weber F, Mirazimi A (2008) Crimean-Congo hemorrhagic fever virus delays activation of the innate immune response. J Med Virol 80(8):1397–1404

    Article  CAS  PubMed  Google Scholar 

  8. Bergeron E, Albarino CG, Khristova ML, Nichol ST (2010) Crimean-Congo hemorrhagic fever virus-encoded ovarian tumor protease activity is dispensable for virus RNA polymerase function. J Virol 84(1):216–226

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  9. Andersson I, Lundkvist A, Haller O, Mirazimi A (2006) Type I interferon inhibits Crimean-Congo hemorrhagic fever virus in human target cells. J Med Virol 78(2):216–222

    Article  CAS  PubMed  Google Scholar 

  10. Morikawa S, Saijo M, Kurane I (2007) Recent progress in molecular biology of Crimean-Congo hemorrhagic fever. Comp Immunol Microbiol Infect Dis 30(5–6):375–389

    Article  PubMed  Google Scholar 

  11. Deyde VM, Khristova ML, Rollin PE, Ksiazek TG, Nichol ST (2006) Crimean-Congo hemorrhagic fever virus genomics and global diversity. J Virol 80(17):8834–8842

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  12. Papadopoulos O, Koptopoulos G (1980) Isolation of the virus from Rhipicephalus bursa ticks and a preliminary serological survey. In: Vesenjak-Hirjan J, Porterfield JS (eds) Arboviruses in the Mediterranean countries. Gustav Fisher Verlag, Stuttgart, pp 117–121

    Google Scholar 

  13. Elevli M, Ozkul AA, Civilibal M, Midilli K, Gargili A, Duru NS (2010) A newly identified Crimean-Congo hemorrhagic fever virus strain in Turkey. Int J Infect Dis 14(Suppl 3):e213–e216

    Article  PubMed  Google Scholar 

  14. Midilli K, Gargili A, Ergonul O, Elevli M, Ergin S, Turan N, Sengoz G, Ozturk R, Bakar M (2009) The first clinical case due to AP92 like strain of Crimean-Congo Hemorrhagic Fever virus and a field survey. BMC Infect Dis 9:90

    Article  PubMed Central  PubMed  Google Scholar 

  15. Duh D, Nichol ST, Khristova ML, Saksida A, Hafner-Bratkovic I, Petrovec M, Dedushaj I, Ahmeti S, Avsic-Zupanc T (2008) The complete genome sequence of a Crimean-Congo hemorrhagic fever virus isolated from an endemic region in Kosovo. Virol J 5:7

    Article  PubMed Central  PubMed  Google Scholar 

  16. Duh D, Saksida A, Petrovec M, Ahmeti S, Dedushaj I, Panning M, Drosten C, Avsic-Zupanc T (2007) Viral load as predictor of Crimean-Congo hemorrhagic fever outcome. Emerg Infect Dis 13(11):1769–1772

    Article  CAS  PubMed  Google Scholar 

  17. Saksida A, Duh D, Wraber B, Dedushaj I, Ahmeti S, Avsic-Zupanc T (2010) Interacting roles of immune mechanisms and viral load in the pathogenesis of crimean-congo hemorrhagic fever. Clin Vaccine Immunol 17(7):1086–1093

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  18. Levine JR, Prescott J, Brown KS, Best SM, Ebihara H, Feldmann H (2010) Antagonism of type I interferon responses by new world hantaviruses. J Virol 84(22):11790–11801. doi:10.1128/JVI.00916-10

    Article  CAS  PubMed Central  PubMed  Google Scholar 

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Acknowledgments

This study was supported by the Slovenian Research Agency (contract no. 1000-10-310162), the European Commission (European Virus Archive, grant agreement no. 228292 and CCH Fever, grant agreement no. 50061). No additional external funding was received for this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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  1. Institute of Microbiology and Immunology, Ljubljana, Slovenia

    Luka Fajs, Katarina Resman & Tatjana Avšič-Županc

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  1. Luka Fajs
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  2. Katarina Resman
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  3. Tatjana Avšič-Županc
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Correspondence to Tatjana Avšič-Županc.

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Fajs, L., Resman, K. & Avšič-Županc, T. Crimean-Congo hemorrhagic fever virus nucleoprotein suppresses IFN-beta-promoter-mediated gene expression. Arch Virol 159, 345–348 (2014). https://doi.org/10.1007/s00705-013-1816-2

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  • DOI: https://doi.org/10.1007/s00705-013-1816-2

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