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Zucchini tigré mosaic virus is a distinct potyvirus in the papaya ringspot virus cluster: molecular and biological insights

Archives of Virology volume 159, pages 277–289 (2014)Cite this article

Abstract

In recent years, three new potyviruses have been described in the papaya ringspot virus (PRSV) cluster. In addition, two types of PRSV are recognized, type W, infecting cucurbit plants, and type P, infecting papaya and also cucurbits. A third type, PRSV-T, was also partially described in Guadeloupe. Complete genome sequencing of four PRSV-T isolates showed that this virus is a related virus that is distinct from PRSV, and the name zucchini tigré mosaic virus (ZTMV) is proposed, in reference to the typical symptoms observed in zucchini squash. Eleven other viral isolates from different geographic origins were confirmed as ZTMV isolates using the complete sequence of the cylindrical inclusion (CI) coding region, whereas pairwise sequence similarities in the coat protein (CP) coding region did not unambiguously distinguish ZTMV isolates from PRSV isolates. The use of the CI coding region for species demarcation appears more suitable than the CP coding region for closely related viruses. Principal coordinates analysis based on the biological behavior of the viral isolates studied clustered PRSV-P, PRSV-W and ZTMV isolates into three different groups. Therefore, ZTMV is different from PRSV in its molecular and biological properties.

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Acknowledgments

We thank Prof. Dan Purcifull and Dr. Carlye Barker for kindly providing reference isolate Q10 of ZTMV. We thank Dr. Michel Pitrat, INRA-Avignon, for kindly providing melon accession seeds for biological tests. This work was partially supported by the cooperation agreement between the Venezuelan Foundation Fundayacucho and the Embassy of France in Venezuela, with a scholarship to GR.

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Affiliations

  1. INRA, UR407 Unité de Pathologie Végétale, 84140, Montfavet, France

    G. Romay, H. Lecoq & C. Desbiez

  2. Fundación Instituto de Estudios Avanzados (IDEA), Carretera Nacional Hoyo de la Puerta Sartenejas, Caracas, 1080, Venezuela

    G. Romay

Authors
  1. G. Romay
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  2. H. Lecoq
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  3. C. Desbiez
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Correspondence to C. Desbiez.

Electronic supplementary material

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705_2013_1798_MOESM1_ESM.docx

Supplementary material 1 (DOCX 14 kb) Supplementary Table S1. Host and geographic origins of viral isolates analyzed in this study

705_2013_1798_MOESM2_ESM.docx

Supplementary material 2 (DOCX 16 kb) Supplementary Table S2. Primers used for amplification and sequencing of ZTMV and PRSV

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Romay, G., Lecoq, H. & Desbiez, C. Zucchini tigré mosaic virus is a distinct potyvirus in the papaya ringspot virus cluster: molecular and biological insights. Arch Virol 159, 277–289 (2014). https://doi.org/10.1007/s00705-013-1798-0

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  • DOI: https://doi.org/10.1007/s00705-013-1798-0

Keywords

  • Coat Protein
  • Zucchini Yellow Mosaic Virus
  • Cylindrical Inclusion
  • Watermelon Mosaic Virus
  • Species Demarcation