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Complete genome sequence of Habenaria mosaic virus, a new potyvirus infecting a terrestrial orchid (Habenaria radiata) in Japan

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Abstract

The complete genomic sequence of Habenaria mosaic virus (HaMV), which infects terrestrial orchids (Habenaria radiata), has been determined. The genome is composed of 9,499 nucleotides excluding the 3′-terminal poly(A) tail, encoding a large polyprotein of 3,054 amino acids with the genomic features typical of a potyvirus. Putative proteolytic cleavage sites were identified by sequence comparison to those of known potyviruses. The HaMV polyprotein showed 58 % amino acid sequence identity to that encoded by the most closely related potyvirus, tobacco vein banding mosaic virus. Phylogenetic analysis of the polyprotein amino acid sequence and its coding sequences confirmed that HaMV formed a cluster with the chilli veinal mottle virus group, most of which infect solanaceous plants. These results suggest that HaMV is a distinct member of the genus Potyvirus.

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Acknowledgments

We are grateful to Kouji Mitsuhata for technical assistance. We thank Dr. Narinobu Inouye for helpful advice. This work was partially supported by a Grant-in-Aid for Scientific Research [KAKENHI] from the Japanese Ministry of Education, Culture, Sport, Science and Technology, and by Yomogi Inc.

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Authors and Affiliations

  1. Institute of Plant Science and Resources (IPSR), Okayama University, Kurashiki, 710-0046, Japan

    Hideki Kondo, I Wayan Gara, Sotaro Chiba, Kazuyuki Maruyama, Tetsuo Tamada & Nobuhiro Suzuki

  2. College of Bioresource Sciences, Nihon University, Fujisawa, 252-8510, Japan

    Takanori Maeda

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  1. Hideki Kondo
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  2. Takanori Maeda
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  3. I Wayan Gara
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  7. Nobuhiro Suzuki
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Correspondence to Hideki Kondo.

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Kondo, H., Maeda, T., Gara, I.W. et al. Complete genome sequence of Habenaria mosaic virus, a new potyvirus infecting a terrestrial orchid (Habenaria radiata) in Japan. Arch Virol 159, 163–166 (2014). https://doi.org/10.1007/s00705-013-1784-6

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  • DOI: https://doi.org/10.1007/s00705-013-1784-6

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