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Characterization of signal sequences determining the nuclear export of Newcastle disease virus matrix protein

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Abstract

The Newcastle disease virus (NDV) matrix (M) protein has been demonstrated to be a nuclear-cytoplasmic trafficking protein. Previous studies have shown that the M protein localizes in the nucleus through a bipartite nuclear localization signal. Here, we report that the ability of the M protein to shuttle to the cytoplasm is mediated by three nuclear export signal sequences (NESs). Using leptomycin B (LMB), a specific inhibitor of CRM1, we found that the nuclear export of the three NESs was LMB insensitive and thus was CRM1 independent. In addition, inactivation of these NESs led to nuclear accumulation of the M protein. Our results highlight the significance of these NESs to the nuclear export of the NDV M protein.

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Fig. 1
Fig. 2

Abbreviations

NDV:

Newcastle disease virus

LMB:

Leptomycin B

CRM1:

Chromosomal region maintenance 1

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Acknowledgments

This work was supported by the Earmarked Fund for Modern Agro-Industry Technology Research System (nycytx-41-G07), the Program for Changjiang Scholars and Innovative Research Team in University (IRT0978), the Priority Academic Program Development of Jiangsu Higher Education Institutions, and Chinese Special Fund for Agro-Scientific Research in the Public Interest (201303033).

Conflict of interest

The authors have declared that no conflict of interest exists.

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Authors and Affiliations

  1. Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, Yangzhou, Jiangsu, China

    Zhiqiang Duan, Qingqing Song, Yuyang Wang, Liang He, Jian Chen, Yanmei Zhu & Shunlin Hu

  2. Animal Infectious Disease Laboratory, College of Veterinary Medicine, Yangzhou University, 12 East Wenhui Road, Yangzhou, 225009, Jiangsu, China

    Xiufan Liu

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  1. Zhiqiang Duan
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  2. Qingqing Song
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  3. Yuyang Wang
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  4. Liang He
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  5. Jian Chen
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  6. Yanmei Zhu
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  7. Shunlin Hu
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Correspondence to Xiufan Liu.

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Duan, Z., Song, Q., Wang, Y. et al. Characterization of signal sequences determining the nuclear export of Newcastle disease virus matrix protein. Arch Virol 158, 2589–2595 (2013). https://doi.org/10.1007/s00705-013-1769-5

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  • DOI: https://doi.org/10.1007/s00705-013-1769-5

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