Abstract
Potato virus S (PVS) (genus Carlavirus, family Betaflexiviridae) is one of the most prevalent viruses in potato crops (Solanum tuberosum and S. phureja) around the world, causing reductions in crop yields between 10 and 20 %. Symptoms of PVS infection may include leaf mottling, rugosity of leaves, deepening of the veins and reductions in crop yields between 10 and 20 %. Virions are flexuous rods of 610-710 nm with a positive-sense ssRNA genome of approximately 8500 nt comprising six ORFs, a 5′CAP and a 3′poly-A tail. PVS has been classified into two groups: PVSO (Ordinary) and PVSA (Andean). PVSA induces severe symptoms in infected plants, such as premature senescence and defoliation, and is more efficiently transmitted by aphids than PVSO. To date, only five PVS genomes have been completely sequenced, including those of three PVSO and two PVSA strains. Currently, there are no reports of complete PVS genome sequences from Andean South America. In this work, we present the complete genomic sequence of a novel PVS strain infecting S. phureja that is clearly distinct from currently known PVS isolates.
Similar content being viewed by others
References
Stevenson WR, Loria R, Franc GD, Weingartner DP (2001) Compendium of potato diseases. APS Press, St. Paul, EEUU
Gil JF, Cotes JM, Marín M (2012) Detección serológica y caracterización molecular de Potato virus S (PVS, Carlavirus) en cultivos de papa de Colombia. Revista Biología Tropical (in press)
Wardrop EA, Gray AB, Singh RP, Peterson JF (1989) Aphid transmission of Potato virus S. Am Potato J 66:449–459
Cox BA, Jones RA (2010) Genetic variability in the coat protein gene of Potato virus S isolates and distinguishing its biologically distinct strains. Arch Virol 155:1163–1169
Matoušek J, Schubert J, Dědič P, Ptáček J (2000) A broad variability of Potato virus S (PVS) revealed by analysis of virus sequences amplified by reverse transcriptase–polymerase chain reaction. Can J Plant Pathol 22:29–37
Matoušek J, Schubert J, Ptáček J, Kozlová P, Dědič P (2005) Complete nucleotide sequence and molecular probing of Potato virus S genome. Acta Virologica 49:195–205
Lambert SJ, Scott J, Pethybridge S, Hay FS (2012) Strain characterization of Potato virus S Isolates from Tasmania, Australia. Plant Dis 96:813–819
Lin Y, Druffel K, Whitworth J, Pavek M, Pappu H (2009) Molecular characterization of two Potato virus S isolates from late-blight-resistant genotypes of potato (Solanum tuberosum). Arch Virol 154:1861–1863
Duarte P, Ferreira S, Rodrigues S, dos Reis A (2012) Complete genome sequence of the first Andean strain of Potato virus S from Brazil and evidence of recombination between PVS strains. Arch Virol 157:1357–1364
Huang X, Madan A (1999) CAP3: A DNA sequence assembly program. Genome Res 9:868–877
Conesa A, Götz S, García-Gómez JM, Terol J, Talón M, Robles M (2005) Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics research. Bioinformatics 21:3674–3676
Milne I, Bayer M, Cardle L, Shaw P, Stephen G, Wright F, Marshall D (2010) Tablet—next generation sequence assembly visualization. Bioinformatics 26:401–402
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evolut 28:2731–2739
Martin DP, Lemey P, Lott M, Moulton V, Posada D, Lefeuvre P (2010) RDP3: a flexible and fast computer program for analyzing recombination. Bioinformatics 26:2462–2463
Salari K, Massumi H, Heydarnejad J, Hosseini A, Varsani A (2011) Analysis of Iranian Potato virus S isolates. Virus Genes 43:281–288
Rodríguez LE, Ñustez CE, Estrada N (2009) Criolla Latina, Criolla Paisa y Criolla Colombia, nuevos cultivares de papa criolla para el departamento de Antioquia (Colombia). Agronomía Colombiana 27(3):289–303
Acknowledgments
This work was funded by Universidad Nacional de Colombia through Grant VRI: 20101009932.
Conflicts of interest
The authors declare that they have no conflict of interest.
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Gutiérrez, P.A., Alzate, J.F. & Marín-Montoya, M.A. Complete genome sequence of a novel potato virus S strain infecting Solanum phureja in Colombia. Arch Virol 158, 2205–2208 (2013). https://doi.org/10.1007/s00705-013-1730-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00705-013-1730-7