Genome sequence analysis of the Vibrio parahaemolyticus lytic bacteriophage VPMS1
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VPMS1 is a Vibrio parahaemolyticus lytic phage isolated from a marine clam. The 42.3-kb genome was predicted to encode 53 proteins. Comparison of the VPMS1 DNA genome with known phage genomes revealed no similarity; hence, it represents a new VP phage, organized into three differently oriented modules. The module for packaging covers 12 % of the genome, the module for structure covers 31 %, and the module for replication and regulation covers 48 %. The G + C content was 44.67 %. The coding region corresponds to 91 % of the genome, and 9 % apparently does not encode any protein. Thirty genes, constituting 57 % of the genome, had significant similarity to some reported proteins in the protein database; 23 genes, constituting 43 % of the genome, showed no significant homology to any reported protein, and these could be new proteins whose hypothetical functions can be deduced from their position in the genome.
KeywordsPhage Genome Lytic Phage Vibrio Parahaemolyticus Hypothetical Function Agar Double Layer
We thank Ira Fogel of Centro de Investigaciones Biológicas del Noroeste, for editorial services, and Diana Barajas for assistance with phage selection, Lina Zermeño for phage production, and Victor Moyrón for technical support. Funding was provided by Consejo Nacional de Ciencia y Tecnología of México (CONACYT grants 52107 and 85033).
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