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Archives of Virology

, Volume 158, Issue 11, pp 2409–2413 | Cite as

Genome sequence analysis of the Vibrio parahaemolyticus lytic bacteriophage VPMS1

  • Martín Ramírez-OrozcoEmail author
  • Vania Serrano-Pinto
  • Norma Ochoa-Álvarez
  • Roman Makarov
  • Sergio F. Martínez-Díaz
Annotated Sequence Record

Abstract

VPMS1 is a Vibrio parahaemolyticus lytic phage isolated from a marine clam. The 42.3-kb genome was predicted to encode 53 proteins. Comparison of the VPMS1 DNA genome with known phage genomes revealed no similarity; hence, it represents a new VP phage, organized into three differently oriented modules. The module for packaging covers 12 % of the genome, the module for structure covers 31 %, and the module for replication and regulation covers 48 %. The G + C content was 44.67 %. The coding region corresponds to 91 % of the genome, and 9 % apparently does not encode any protein. Thirty genes, constituting 57 % of the genome, had significant similarity to some reported proteins in the protein database; 23 genes, constituting 43 % of the genome, showed no significant homology to any reported protein, and these could be new proteins whose hypothetical functions can be deduced from their position in the genome.

Keywords

Phage Genome Lytic Phage Vibrio Parahaemolyticus Hypothetical Function Agar Double Layer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We thank Ira Fogel of Centro de Investigaciones Biológicas del Noroeste, for editorial services, and Diana Barajas for assistance with phage selection, Lina Zermeño for phage production, and Victor Moyrón for technical support. Funding was provided by Consejo Nacional de Ciencia y Tecnología of México (CONACYT grants 52107 and 85033).

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Copyright information

© Springer-Verlag Wien 2013

Authors and Affiliations

  • Martín Ramírez-Orozco
    • 1
    • 2
    Email author
  • Vania Serrano-Pinto
    • 1
  • Norma Ochoa-Álvarez
    • 1
  • Roman Makarov
    • 2
  • Sergio F. Martínez-Díaz
    • 2
  1. 1.Centro de Investigaciones Biológicas del Noroeste (CIBNOR)Instituto Politécnico NacionalLa PazMexico
  2. 2.Centro Interdisciplinario de Ciencias Marinas-IPN (CICIMAR)Av. Instituto Politécnico NacionalLa PazMexico

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