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MicroRNA-21 and -146b are involved in the pathogenesis of murine viral myocarditis by regulating TH-17 differentiation

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Abstract

Viral myocarditis (VMC) is a common cardiovascular disease, and microRNAs (miRNAs) have been postulated to be involved in its pathology. Using microarrays, we observed that miRNA-21 and -146b were upregulated in a murine model of VMC. We also found that miRNA-451 was downregulated. In vivo silencing of miRNA-21 and -146b resulted in less-severe VMC. Overexpression of miRNA-451 did not ameliorate the severity of VMC. Further work revealed that inhibition of miRNA-21 and -146b decreased the expression levels of Th17 and RORγt . Overexpression of miRNA-451 had no effect on IL-17 and RORγt expression. Inhibition of miRNA-21 and -146b might ameliorate myocardium inflammation by mediating downregulation of RORγt expression, indicating that these miRNAs are involved in the pathogenesis of murine VMC.

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Acknowledgments

We thank Dr. Jiao Lan, Xiu-Jiu Liang, and Qi-Guang Huang for their technical assistance. LY coordinated the study, carried out data collection, performed the statistical analysis, interpreted data, and drafted the manuscript. WW participated in the conception and design of the study, coordinated the study, and reviewed the manuscript. XY, GM, YY, KQ, PY and YF carried out data collection. All authors read and approved the final manuscript. This work was supported by grants from the National Natural Science Foundation of China (No. 30960129).

Conflict of interest

The authors declare that they have no competing interests, and had no conflicts of interest to declare.

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Authors and Affiliations

  1. Guangxi Cardiovascular Institute, Shuang-Yong Road 6, Nanning, 530021, People’s Republic of China

    Yan Li Liu & WeiFeng Wu

  2. Department of Cardiology, The First Affiliated Hospital of Guangxi Medical University, Shuang-Yong Road 6, Nanning, People’s Republic of China

    Yan Li Liu & WeiFeng Wu

  3. Guangxi Medical University, Shuang-Yong Road 6, Nanning, People’s Republic of China

    Yimin Xue, Mengsha Gao, Yuluan Yan, Qing Kong & Yu Pang

  4. The Fourth Affiliated Hospital of Guangxi Medical University, Liu-Shi Road 1, Liuzhou, 545005, People’s Republic of China

    Fan Yang

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  1. Yan Li Liu
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Correspondence to WeiFeng Wu.

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Liu, Y.L., Wu, W., Xue, Y. et al. MicroRNA-21 and -146b are involved in the pathogenesis of murine viral myocarditis by regulating TH-17 differentiation. Arch Virol 158, 1953–1963 (2013). https://doi.org/10.1007/s00705-013-1695-6

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  • DOI: https://doi.org/10.1007/s00705-013-1695-6

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