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Molecular detection of Aichi virus in raw sewage in Italy

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Abstract

Aichi virus (AiV) is suspected to play a role in viral gastroenteritis in humans. In this study, we assessed the presence of AiV in untreated influent sewage samples collected at four wastewater treatment plants in central Italy. AiV was detected in 6 (12.5 %) of the 48 specimens and in all plants. All of the Italian strains showed the highest nucleotide and amino acid sequence identity to genotype B AiV detected recently in Asia, especially in China.

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References

  1. Alcalá A, Vizzi E, Rodríguez-Díaz J, Zambrano JL, Betancourt W, Liprandi F (2010) Molecular detection and characterization of Aichi viruses in sewage-polluted waters of Venezuela. Appl Environ Microbiol 76:4113–4115

    Article  PubMed  Google Scholar 

  2. Ambert-Balay K, Lorrot M, Bon F, Giraudon H, Kaplon J, Wolfer M, Lebon P, Gendrel D, Pothier P (2008) Prevalence and genetic diversity of Aichi virus strains in stool samples from community and hospitalized patients. J Clin Microbiol 46:1252–1258

    Article  PubMed  CAS  Google Scholar 

  3. Blinkova O, Rosario K, Li L, Kapoor A, Slikas B, Bernardin F, Breitbart M, Delwart E (2009) Frequent detection of highly diverse variants of cardiovirus, cosavirus, bocavirus, and circovirus in sewage samples collected in the United States. J Clin Microbiol 47:3507–3513

    Article  PubMed  CAS  Google Scholar 

  4. Drexler JF, Baumgarte S, de Souza Luna LK, Eschbach-Bludau M, Lukashev AN, Drosten C (2011) Aichi virus shedding in high concentrations in patients with acute diarrhea. Emerg Infect Dis 17:1544–1548

    PubMed  Google Scholar 

  5. Drexler JF, Grywna K, Stöcker A, Almeida PS, Medrado-Ribeiro TC, Eschbach-Bludau M, Petersen N, da Costa-Ribeiro HJ, Drosten C (2009) Novel human parechovirus from Brazil. Emerg Infect Dis 15:310–313

    Article  PubMed  CAS  Google Scholar 

  6. Goyer M, Aho LS, Bour JB, Ambert-Balay K, Pothier P (2008) Seroprevalence distribution of Aichi virus among a French population in 2006–2007. Arch Virol 153:1171–1174

    Article  PubMed  CAS  Google Scholar 

  7. Jonsson N, Wahlström K, Svensson L, Serrander L, Lindberg AM (2012) Aichi virus infection in elderly people in Sweden. Arch Virol 157:1365–1369

    Article  PubMed  CAS  Google Scholar 

  8. Kaikkonen S, Rasanen S, Ramet M, Vesikari T (2010) Aichi virus infection in children with acute gastroenteritis in Finland. Epidemiol Infect 138:1166–1171

    Article  PubMed  CAS  Google Scholar 

  9. Knowles NJ, Hovi T, Hyypiä T, King AMQ, Lindberg AM, Pallansch MA, Palmenberg AC, Simmonds P, Skern T, Stanway G, Yamashita T, Zell R (2012) Picornaviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (ed) Virus taxonomy: classification and nomenclature of viruses: ninth report of the international committee on taxonomy of viruses. San Diego, Elsevier, pp 855–880

  10. Kumar S, Tamura K, Nei M (2004) MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:153–163

    Article  Google Scholar 

  11. Le Guyader FS, Le Saux JC, Ambert-Balay K, Krol J, Serais O, Parnaudeau S, Giraudon H, Delmas G, Pommepuy M, Pothier P, Atmar RL (2008) Aichi virus, norovirus, astrovirus, enterovirus, and rotavirus involved in clinical cases from a French oyster related gastroenteritis outbreak. J Clin Microbiol 46:4011–4017

    Article  PubMed  Google Scholar 

  12. Li L, Victoria J, Kapoor A, Blinkova O, Wang C, Babrzadeh F, Mason CJ, Pandey P, Triki H, Bahri O, Oderinde BS, Baba MM, Bukbuk DN, Besser JM, Bartkus JM, Delwart EL (2009) A novel picornavirus associated with gastroenteritis. J Virol 83:12002–12006

    Article  PubMed  CAS  Google Scholar 

  13. Oh DY, Silva PA, Hauroeder B, Diedrich S, Cardoso DD, Schreier E (2006) Molecular characterization of the first Aichi viruses isolated in Europe and in South America. Arch Virol 151:1199–1206

    Article  PubMed  CAS  Google Scholar 

  14. Pham NT, Khamrin P, Nguyen TA, Kanti DS, Phan TG, Okitsu S, Ushijima H (2007) Isolation and molecular characterization of Aichi viruses from fecal specimens collected in Japan, Bangladesh, Thailand, and Vietnam. J Clin Microbiol 45:2287–2288

    Article  PubMed  CAS  Google Scholar 

  15. Pringle CR (1999) Virus taxonomy at the XIth international congress of virology, Sydney, Australia, 1999. Arch Virol 144:2065–2070

    Article  PubMed  CAS  Google Scholar 

  16. Reuter G, Boldizsár A, Pankovics P (2009) Complete nucleotide and amino acid sequences and genetic organization of porcine kobuvirus, a member of a new species in the genus Kobuvirus, family Picornaviridae. Arch Virol 154:101–108

    Article  PubMed  CAS  Google Scholar 

  17. Reuter G, Boldizsar A, Papp G, Pankovics P (2009) Detection of Aichi virus shedding in a child with enteric and extraintestinal symptoms in Hungary. Arch Virol 154:1529–1532

    Article  PubMed  CAS  Google Scholar 

  18. Reuter G, Boros A, Pankovics P (2011) Kobuviruses—a comprehensive review. Rev Med Virol 21:32–41

    Article  PubMed  Google Scholar 

  19. Ribes JM, Montava R, Tellez-Castillo CJ, Fernandez-Jimenez M, Buesa J (2010) Seroprevalence of Aichi virus in a Spanish population from 2007 to 2008. Clin Vaccine Immunol 17:545–549

    Article  PubMed  CAS  Google Scholar 

  20. Sasaki J, Kusuhara Y, Maeno Y, Kobayashi N, Yamashita T, Sakae K, Takeda N, Taniguchi K (2001) Construction of an infectious cDNA clone of Aichi virus (a new member of the family Picornaviridae) and mutational analysis of a stem-loop structure at the 5′ end of the genome. J Virol 75:8021–8030

    Article  PubMed  CAS  Google Scholar 

  21. Sdiri-Loulizi K, Hassine M, Aouni Z, Gharbi-Khelifi H, Sakly N, Chouchane S, Guediche MN, Pothier P, Aouni M, Ambert-Balay K (2010) First molecular detection of Aichi virus in sewage and shellfish samples in the Monastir region of Tunisia. Arch Virol 155:1509–1513

    Article  PubMed  CAS  Google Scholar 

  22. Sdiri-Loulizi K, Hassine M, Bour JB, Ambert-Balay K, Mastouri M, Aho LS, Gharbi-Khelifi H, Aouni Z, Sakly N, Chouchane S, Neji-Guediche M, Pothier P, Aouni M (2010) Aichi virus IgG seroprevalence in Tunisia parallels genomic detection and clinical presentation in children with gastroenteritis. Clin Vaccine Immunol 17:1111–1116

    Article  PubMed  CAS  Google Scholar 

  23. Sdiri-Loulizi K, Hassine M, Gharbi-Khelifi H, Sakly N, Chouchane S, Guediche MN, Pothier P, Aouni M, Ambert-Balay K (2009) Detection and genomic characterization of Aichi viruses in stool samples from children in Monastir, Tunisia. J Clin Microbiol 47:2275–2278

    Article  PubMed  Google Scholar 

  24. Yamashita T, Ito M, Kabashima Y, Tsuzuki H, Fujiura A, Sakae K (2003) Isolation and characterization of a new species of kobuvirus associated with cattle. J Gen Virol 84:3069–3077

    Article  PubMed  CAS  Google Scholar 

  25. Yamashita T, Kobayashi S, Sakae K, Nakata S, Chiba S, Ishihara Y, Isomura S (1991) Isolation of cytopathic small round viruses with BS-C-1 cells from patients with gastroenteritis. J Infect Dis 164:954–957

    Article  PubMed  CAS  Google Scholar 

  26. Yamashita T, Sakae K, Ishihara Y, Isomura S, Utagawa E (1993) Prevalence of newly isolated, cytopathic small round virus (Aichi strain) in Japan. J Clin Microbiol 31:2938–2943

    PubMed  CAS  Google Scholar 

  27. Yamashita T, Sakae K, Tsuzuki H, Suzuki Y, Ishikawa N, Takeda N, Miyamura T, Yamazaki S (1998) Complete nucleotide sequence and genetic organization of Aichi virus, a distinct member of the Picornaviridae associated with acute gastroenteritis in humans. J Virol 72:8408–8412

    PubMed  CAS  Google Scholar 

  28. Yamashita T, Sugiyama M, Tsuzuki H, Sakae K, Suzuki Y, Miyazaki Y (2000) Application of a reverse transcription-PCR for identification and differentiation of Aichi virus, a new member of the Picornavirus family associated with gastroenteritis in humans. J Clin Microbiol 38:2955–2961

    PubMed  CAS  Google Scholar 

  29. Yang S, Zhang W, Shen Q, Yang Z, Zhu J, Cui L, Hua X (2009) Aichi virus strains in children with gastroenteritis, China. Emerg Infect Dis 15:1703–1705

    Article  PubMed  CAS  Google Scholar 

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Acknowledgements

We thank the Ruzzo Reti SpA for its technical support during sewage sample collection. This work was financed by grants from the University of Teramo, Italy, and from the Italian Ministry of University and Research.

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All of the authors declare that there are no financial or other relationships that might lead to a conflict of interest. All of the authors have seen and approved the manuscript and have contributed significantly to the work.

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Correspondence to Barbara Di Martino.

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Di Martino, B., Di Profio, F., Ceci, C. et al. Molecular detection of Aichi virus in raw sewage in Italy. Arch Virol 158, 2001–2005 (2013). https://doi.org/10.1007/s00705-013-1694-7

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  • DOI: https://doi.org/10.1007/s00705-013-1694-7

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