Skip to main content

Advertisement

SpringerLink
Log in

Genomic sequence analysis of a new reassortant infectious bursal disease virus from commercial broiler flocks in central China

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

We report the complete nucleotide sequence of a reassortant infectious bursal disease (IBD) virus (IBDV) HN isolate from commercial broiler flocks in central China. The genome consisted of 3,232 and 2,652 nucleotides in the coding regions of segments A and B, respectively. Alignment of both nucleotide and deduced amino acid sequences and phylogenetic analysis revealed that the genome segments A and B of HN were derived from the attenuated strain B87 and the VV strain OKYM. This is a new reassortant IBDV strain that has emerged in nature, involving segment A of a cell-culture-adapted attenuated vaccine strain B87.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

References

  1. Chen F, Liu J, Yan Z, Liu D, Ji J, Qin J, Li H, Ma J, Bi Y, Xie Q (2012) Complete genome sequence analysis of a natural reassortant infectious bursal disease virus in China. J Virol 86:11942–11943

    Article  PubMed  CAS  Google Scholar 

  2. Da Costa B, Chevalier C, Henry C, Huet JC, Petit S, Lepault J, Boot H, Delmas B (2002) The capsid of infectious bursal disease virus contains several small peptides arising from the maturation process of pVP2. J Virol 76:2393–2402

    Article  PubMed  Google Scholar 

  3. Gao HL, Wang XM, Gao YL, Fu CY (2007) Direct evidence of reassortment and mutant spectrum analysis of a very virulent infectious bursal disease virus. Avian Dis 51:893–899

    Article  PubMed  Google Scholar 

  4. He X, Wei P, Yang X, Guan D, Wang G, Qin A (2012) Molecular epidemiology of infectious bursal disease viruses isolated from Southern China during the years 2000–2010. Virus Genes 45:246–255

    Article  PubMed  CAS  Google Scholar 

  5. Hon CC, Lam TY, Drummond A, Rambaut A, Lee YF, Yip CW, Zeng F, Lam PY, Ng PT, Leung FC (2006) Phylogenetic analysis reveals a correlation between the expansion of very virulent infectious bursal disease virus and reassortment of its genome segment B. J Virol 80:8503–8509

    Article  PubMed  CAS  Google Scholar 

  6. Jackwood DJ, Sommer-Wagner SE, Crossley BM, Stoute ST, Woolcock PR, Charlton BR (2011) Identification and pathogenicity of a natural reassortant between a very virulent serotype 1 infectious bursal disease virus (IBDV) and a serotype 2 IBDV. Virology 420:98–105

    Article  PubMed  CAS  Google Scholar 

  7. Jackwood DJ (2012) Molecular epidemiologic evidence of homologous recombination in infectious bursal disease viruses. Avian Dis 56:574–577

    Article  PubMed  Google Scholar 

  8. Kasanga CJ, Yamaguchi T, Munang’andu HM, Ohya K, Fukushi H (2012) Genomic sequence of an infectious bursal disease virus isolate from Zambia: classical attenuated segment B reassortment in nature with existing very virulent segment A. Arch Virol 6:6

    Google Scholar 

  9. Le Nouen C, Rivallan G, Toquin D, Darlu P, Morin Y, Beven V, de Boisseson C, Cazaban C, Comte S, Gardin Y, Eterradossi N (2006) Very virulent infectious bursal disease virus: reduced pathogenicity in a rare natural segment-B-reassorted isolate. J Gen Virol 87:209–216

    Article  PubMed  Google Scholar 

  10. Lejal N, Da Costa B, Huet JC, Delmas B (2000) Role of Ser-652 and Lys-692 in the protease activity of infectious bursal disease virus VP4 and identification of its substrate cleavage sites. J Gen Virol 81:983–992

    PubMed  CAS  Google Scholar 

  11. Liu D, Zhang XB, Yan ZQ, Chen F, Ji J, Qin JP, Li HY, Lu JP, Xue Y, Liu JJ, Xie QM, Ma JY, Xue CY, Bee YZ (2012) Molecular characterization and phylogenetic analysis of infectious bursal disease viruses isolated from chicken in South China in 2011. Trop Anim Health Prod 2:2

    Google Scholar 

  12. Liu M, Vakharia VN (2004) VP1 protein of infectious bursal disease virus modulates the virulence in vivo. Virology 330:62–73

    Article  PubMed  CAS  Google Scholar 

  13. McNulty MS, Allan GM, Todd D, McFerran JB, McKillop ER, Collins DS, McCracken RM (1980) Isolation of rotaviruses from turkeys and chickens: demonstration of distinct serotypes and RNA electropherotypes. Avian Pathol 9:363–375

    Article  PubMed  CAS  Google Scholar 

  14. Muller H, Scholtissek C, Becht H (1979) The genome of infectious bursal disease virus consists of two segments of double-stranded RNA. J Virol 31:584–589

    PubMed  CAS  Google Scholar 

  15. Muller H, Islam MR, Raue R (2003) Research on infectious bursal disease–the past, the present and the future. Vet Microbiol 97:153–165

    Article  PubMed  Google Scholar 

  16. Sharma JM, Kim IJ, Rautenschlein S, Yeh HY (2000) Infectious bursal disease virus of chickens: pathogenesis and immunosuppression. Dev Comp Immunol 24:223–235

    Article  PubMed  CAS  Google Scholar 

  17. Shwed PS, Dobos P, Cameron LA, Vakharia VN, Duncan R (2002) Birnavirus VP1 proteins form a distinct subgroup of RNA-dependent RNA polymerases lacking a GDD motif. Virology 296:241–250

    Article  PubMed  CAS  Google Scholar 

  18. Singh A, Bedekar MK, Sharma R, Sarkhel BC, Singh S, Jain SK (2012) Detection of very virulent infectious bursal disease virus from a field outbreak in Central India. Acta Vet Hung 60:165–174

    Article  PubMed  CAS  Google Scholar 

  19. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    Article  PubMed  CAS  Google Scholar 

  20. Wang YS, Wang ZC, Tang YD, Shi ZL, He KW, Li Y, Hou JB, Yao HC, Fan HJ, Lu CP (2007) Comparison of four infectious bursal disease viruses isolated from different bird species. Arch Virol 152:1787–1797

    Article  PubMed  CAS  Google Scholar 

  21. Wei Y, Li J, Zheng J, Xu H, Li L, Yu L (2006) Genetic reassortment of infectious bursal disease virus in nature. Biochem Biophys Res Commun 350:277–287

    Article  PubMed  CAS  Google Scholar 

  22. Wei Y, Yu X, Zheng J, Chu W, Xu H, Yu L (2008) Reassortant infectious bursal disease virus isolated in China. Virus Res 131:279–282

    Article  PubMed  CAS  Google Scholar 

  23. Wu CC, Rubinelli P, Lin TL (2007) Molecular detection and differentiation of infectious bursal disease virus. Avian Dis 51:515–526

    Article  PubMed  Google Scholar 

  24. Xia RX, Wang HY, Huang GM, Zhang MF (2008) Sequence and phylogenetic analysis of a Chinese very virulent infectious bursal disease virus. Arch Virol 153:1725–1729

    Article  PubMed  CAS  Google Scholar 

  25. Yuwen Y, Gao Y, Gao H, Qi X, Li T, Liu W, Wang X (2008) Sequence analysis of the VP2 hypervariable region of eight very virulent infectious bursal disease virus isolates from the northeast of China. Avian Dis 52:284–290

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (30671561) and the Henan Provincial Natural Science Foundation (092300410097).

Author information

Authors and Affiliations

  1. College of Animal Science and Veterinary Medicine, Henan Agricultural University, Nongye Road 63#, 450002, Zhengzhou, Henan Province, People’s Republic of China

    Shi-Jie Ma, Yu-Geng Zhang, Xin-Sheng Li, Xiao-Yun Gao, Bao-An Cui & Hong-Ying Chen

  2. Henan Center for Animal Disease Control and Prevention, Animal Husbandry Bureau of Henan Province, Zhengzhou, 450008, Henan Province, People’s Republic of China

    Pei Cui

Authors
  1. Pei Cui
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shi-Jie Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yu-Geng Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xin-Sheng Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xiao-Yun Gao
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Bao-An Cui
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Hong-Ying Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hong-Ying Chen.

Additional information

P. Cui and S.-J. Ma contributed equally to this work.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cui, P., Ma, SJ., Zhang, YG. et al. Genomic sequence analysis of a new reassortant infectious bursal disease virus from commercial broiler flocks in central China. Arch Virol 158, 1973–1978 (2013). https://doi.org/10.1007/s00705-013-1682-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-013-1682-y

Keywords

Search

Navigation