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Characterization and genome sequencing of a novel bacteriophage infecting Streptococcus agalactiae with high similarity to a phage from Streptococcus pyogenes

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Abstract

A novel bacteriophage, JX01, specifically infecting bovine Streptococcus agalactiae was isolated from milk of mastitis-affected cattle. The phage morphology showed that JX01 belongs to the family Siphoviridae, and this phage demonstrated a broad host range. Microbiological characterization demonstrated that nearly 90 % of JX01 phage particles were adsorbed after 2.5 min of incubation, that the burst size was 20 virions released per infected host cell, and that there was a latent period of 30 min. JX01 was thermal sensitive and showed acid and alkaline resistance (pH 3–11). The genome of JX01 was found to consist of a linear, double-stranded 43,028-bp DNA molecule with a GC content of 36.81 % and 70 putative open reading frames (ORFs) plus one tRNA. Comparative genome analysis revealed high similarity between JX01 and the prophage 315.2 of Streptococcus pyogenes.

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Acknowledgments

We thank Drs. Moineau and Tillett for their support and suggestions during the preparation of this manuscript. This work was supported by grants from the Program for New Century Excellent Talents in University of Ministry of Education of China (NCET-110671), and the Priority Academic Program Development of Jiangsu Higher Education Institutions.

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Authors and Affiliations

  1. Key Lab of Animal Bacteriology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China

    Qinqin Bai, Wei Zhang, Yongchun Yang, Fang Tang, Xuanhoa Nguyen, Guangjin Liu & Chengping Lu

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  1. Qinqin Bai
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  2. Wei Zhang
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  3. Yongchun Yang
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  4. Fang Tang
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  7. Chengping Lu
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Correspondence to Chengping Lu.

Additional information

Nucleotide sequence data reported are available in the GenBank database under the accession number JX409895.

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Bai, Q., Zhang, W., Yang, Y. et al. Characterization and genome sequencing of a novel bacteriophage infecting Streptococcus agalactiae with high similarity to a phage from Streptococcus pyogenes . Arch Virol 158, 1733–1741 (2013). https://doi.org/10.1007/s00705-013-1667-x

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