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HBx and HBs regulate RhoC expression by upregulating transcription factor Ets-1

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Abstract

The available evidence suggests that HBV proteins play an important role in the development of hepatocellular carcinoma (HCC). RhoC, a member of the Rho subfamily of the Ras superfamily of homologous genes, had been implicated in tumorigenesis and tumor progression. In a previous study, we demonstrated that HBx and HBs could up-regulate RhoC expression by enhancing its promoter activity. However, the specific mechanisms remain unclear. Here, we demonstrate that overexpression of Ets-1 results in upregulation of RhoC promoter activity and mRNA and protein levels. Expression of transcription factor Ets-1 was significantly higher in HepG2.2.15 cells than that in HepG2 cells. Meanwhile, infection of HepG2 cells with an HBV-adenovirus recombinant virus led to up-regulation of Ets-1. Of the four HBV proteins, HBx and HBs, could increase expression of Ets-1, which consequently contributed to the upregulation of RhoC. These findings might provide a novel insight into HBV-induced HCC metastasis.

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Acknowledgment

This work was supported by Nature Science Foundation of China (30771924) and Natural Science Foundation Project of CQ CSTC (2010BB5359).

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Authors and Affiliations

  1. Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Second Affiliated Hospital, Chongqing Medical University, 400016, Chongqing, China

    Dongdong Qin, Kai Li, Jialin Qu, Sen Wang, Chengcheng Zou, Yanrui Sheng, Ailong Huang & Hua Tang

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  1. Dongdong Qin
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  2. Kai Li
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  3. Jialin Qu
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  4. Sen Wang
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  7. Ailong Huang
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  8. Hua Tang
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Correspondence to Hua Tang.

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D. Qin, K. Li contributed equally to this work.

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Qin, D., Li, K., Qu, J. et al. HBx and HBs regulate RhoC expression by upregulating transcription factor Ets-1. Arch Virol 158, 1773–1781 (2013). https://doi.org/10.1007/s00705-013-1655-1

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