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Characterization of the complete genome of a novel citrivirus infecting Actinidia chinensis

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Abstract

A ssRNA virus from kiwifruit (Actinidia spp.) was identified as a member of the family Betaflexiviridae. It was mechanically transmitted to the herbaceous indicators Nicotiana benthamiana, N. clevelandii, N. glutinosa and N. occidentalis. The complete genome was comprised of three ORFs and a 3’poly (A) tail. Phylogenetic analysis of the entire genome indicated it was a novel member of the genus Citrivirus (family Betaflexiviridae). The complete nucleotide sequence differed from that of citrus leaf blotch virus (CLBV) by ~ 26 %. The movement protein (ORF2) and coat protein (ORF3) shared 95-96 % and 90-92 % amino acid sequence identity, respectively, with CLBV. The replicase polyprotein (ORF1) was distinctly different from published CLBV sequences, with 78-79 % amino acid sequence identity, while the 5’ UTR and 3’ UTR differed from CLBV by 28 % and 29 %, respectively. The sequence differences indicate that the citrivirus from Actinidia is either a divergent strain of CLBV or a member of a new citrivirus species.

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References

  1. Adams MJ, Candresse T, Hammond J, Kreuze JF, Martelli GP, Namba S, Pearson MN, Ryu KH, Saldarelli P, Yoshikawa N (2012) Family Betaflexiviridae 920–941. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus Taxonomy: Ninth Report of the International Committee on Taxonomy of Viruses. Elsevier Academic Press, Amsterdam

  2. Aravind L, Koonin EV (2001) The DNA-repair protein AlkB, EGL-9, and leprecan define new families of 2-oxoglutarate-and iron-dependent dioxygenases. Genome Biol 2: research0007.1–research0007.8

  3. Marais A, Faure C, Gentit P, Foissac X, Candresse T (2010) Molecular characterization of a new prunus-infecting flexiviridae member. 21st international conference on virus and other graft transmissible deseases of fruit. Crops Julius-Kühn-Archiv 427:37

    Google Scholar 

  4. Blouin AG, Chavan RR, Pearson MN, MacDiarmid RM, Cohen D (2012) Detection and characterisation of two novel vitiviruses infecting Actinidia. Arch Virol 157:713–722

    Article  PubMed  CAS  Google Scholar 

  5. Chavan RR, Pearson MN, Cohen D (2009) Partial characterization of a novel Tobamovirus infecting Actinidia chinensis and A. deliciosa (Actinidiaceae) from China. Eur J Plant Pathol 124:247–259

    Article  Google Scholar 

  6. Chavan RR, Cohen D, Blouin AG, Pearson MN (2012) Characterization of the complete genome of ribgrass mosaic virus isolated from Plantago major L. from New Zealand and Actinidia spp. from China. Arch Virol 157:1253–1260

    Article  PubMed  CAS  Google Scholar 

  7. Clover GRG, Pearson MN, Elliott DR, Tang Z, Smales TE, Alexander BJR (2003) Characterization of a strain of Apple stem grooving virus in Actinidia chinensis from China. Plant Pathol 52:371–378

    Article  CAS  Google Scholar 

  8. Ferguson AR, Bollard EG (1990) Domestication of the kiwifruit. In: Warrington IJ, Weston GC (eds) Kiwifruit: Science and Management. Ray Richards in asscociation with the New Zealand Society for Horticultural Science, Auckland, pp 165–246

  9. Galipienso L, Navarro L, Ballester-Olmos JF, Pina J, Moreno P, Guerri J (2000) Host range and symptomatology of graft-transmissible pathogen causing bud union crease of citrus on trifoliate rootstocks. Plant Pathol 49:308–314

    Article  Google Scholar 

  10. Galipienso L, Vives MC, Moreno P, Milne RG, Navarro L, Guerri J (2001) Partial characterization of Citrus leaf blotch virus, a new virus from Nagami kumquat. Arch Virol 146:357–368

    Article  PubMed  CAS  Google Scholar 

  11. Guardo M, Potere O, Castellano MA, Savino V, Caruso A (2009) A new herbaceous host of Citrus leaf blotch virus. J Plant Path 91:485–488

    CAS  Google Scholar 

  12. Guerri J, Pina JA, Vives MC, Navarro L, Moreno P (2004) Seed transmission of Citrus leaf botch virus: Implications in quarantine and certification programs. Plant Dis 88:906

    Article  Google Scholar 

  13. Hajeri S, Ramadugu C, Keremane M, Vidalakis G, Lee R (2010) Nucleotide sequence and genome organization of Dweet mottle virus and its relationship to members of the family Betaflexiviridae. Arch Virol 155:1523–1527

    Article  PubMed  CAS  Google Scholar 

  14. Komatsu K, Yamaji Y, Ozeki J, Hashimoto M, Kagiwada S, Takahashi S, Namba S (2008) Nucleotide sequence analysis of seven Japanese isolates of Plantago asiatica mosaic virus (PlAMV): a unique potexvirus with significantly high genomic and biological variability within the species. Arch Virol 153:193–198

    Article  PubMed  CAS  Google Scholar 

  15. Li X-W, Li J-Q, Soejarto DD (2009) Advances in the study of the systematics of Actinidia Lindley. Front Biol Chin 4:55–61

    Article  Google Scholar 

  16. Lole KS, Bollinger RC, Paranjape RS, Gadkari D, Kulkarni SS, Novak NG, Ingersoll R, Sheppard HW, Ray SC (1999) Full-length human immunodeficiency virus type 1 genomes from subtype C-infected seroconverters in India, with evidence of intersubtype recombination. J Virol 73(1):152–160

    PubMed  CAS  Google Scholar 

  17. Martelli GP, Adams MJ, Kreuze JF, Dolja VV (2007) Family Flexiviridae: A case study in virion and genome plasticity. Ann Rev Phytopath 45:73–100

    Article  CAS  Google Scholar 

  18. Navarro L, Pina JA, Ballester-Olmos JF, Moreno P, Cambra M (1984) A new graft transmissible disease found in Nagami kumquat. In: Proceeding 9th Conf Int Org Citrus Virologists, Riverside, pp 234–240

  19. Pearson MN, Chavan RR, Cohen D (2007) Viruses of Actinidia: do they pose a threat to kiwifruit production? Acta Hort 753:639–644

    CAS  Google Scholar 

  20. Pearson MN, Cohen D, Chavan RR, Blouin AG, Cowell SJ (2010) Molecular characterisation of viruses from kiwifruit. 21st int conf virus and other graft transmissible diseases of fruit crops. Julius-Kühn-Archiv 427:87–91

    Google Scholar 

  21. Roistacher CN, Blue RL (1968) A psorosis-like virus causing symptoms only on Dweet tangor. In: Proceeding 4th Conf Intl Org Citrus Virologists, Gainesville, pp 13–18

  22. Roistacher CN, Nauer EM, Wagner RC (1980) Transmissibility of cachexia, Dweet mottle, psorosis and infectious variegation viruses on knife blades and its prevention. In: Proceeding 8th Conf Int Org Citrus Virologists, Riverside, pp 225–229

  23. Sweet JB (1975) Soil-borne viruses occurring in nursery soils and infecting some ornamental species of Rosaceae. Ann Appl Biol 79:49–54. doi:10.1111/j.1744-7348.1975.tb01521.x

    Article  PubMed  CAS  Google Scholar 

  24. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Bio Evol 28:2731–2739

    Article  CAS  Google Scholar 

  25. Vives MC, Galipienso L, Navarro L, Moreno P, Guerri J (2001) The nucleotide sequence and genomic organization of citrus leaf blotch virus: candidate type species for a new virus genus. Virology 287:225–233

    Article  PubMed  CAS  Google Scholar 

  26. Vives MC, Galipienso L, Navarro L, Moreno P, Guerri J (2002) Citrus leaf blotch virus: a new citrus virus associated with bud union crease on trifoliate rootstocks. In: Proceeding 15th Conf Int Org Citrus Virologists, Riverside, pp 205–212

  27. Vives MC, Pina JA, Juarez J, Navarro L, Moreno P, Guerri J (2005) Dweet mottle disease is probably caused by Citrus leaf blotch virus. In: Proceeding 16th Conf Int Org Citrus Virologists, Riverside, pp 251–256

  28. Vives MC, Martìn S, Ambrus S, Renovell A, Navarro L, Pina JA, Moreno P, Guerri J (2008) Development of a full genome cDNA clone of Citrus leaf blotch virus and infection of citrus plants. Mol Plant Path 9:787–797

    Article  CAS  Google Scholar 

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Acknowledgments

The authors wish to thank Zespri Innovation and Plant and Food Research for funding this work, and Jane Lancaster and Peter Berry (Zespri Innovation) for their management of the quarantine plants and associated testing programme.

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Authors and Affiliations

  1. School of Biological Sciences, The University of Auckland, Private Bag 92019, Auckland, New Zealand

    Ramesh R. Chavan & Michael N. Pearson

  2. The New Zealand Institute for Plant & Food Research Ltd, Private Bag 92169, Auckland, New Zealand

    Arnaud G. Blouin & Daniel Cohen

Authors
  1. Ramesh R. Chavan
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  2. Arnaud G. Blouin
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  3. Daniel Cohen
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  4. Michael N. Pearson
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Correspondence to Michael N. Pearson.

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Chavan, R.R., Blouin, A.G., Cohen, D. et al. Characterization of the complete genome of a novel citrivirus infecting Actinidia chinensis . Arch Virol 158, 1679–1686 (2013). https://doi.org/10.1007/s00705-013-1654-2

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  • DOI: https://doi.org/10.1007/s00705-013-1654-2

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