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Complete genome sequence of ΦCP51, a temperate bacteriophage of Clostridium perfringens

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Abstract

During sequencing of the genome of Clostridium perfringens strain 5147-97, a putative prophage was identified, located within a gene for a proposed flavodoxin oxidoreductase. Mitomycin C induction of this strain released a bacteriophage whose morphological features examined by electron microscopy indicated it belonged to the family Siphoviridae. This phage was hence designated as vB_CpeS-CP51. The 39,108-bp genome includes 50 predicted open reading frames (ORFs), including two that may affect sporulation, and two predicted tRNAs. To determine the ends of the prophage, PCR was performed using primers facing outwards from the proposed end genes. This confirmed the presence of a circularised genome in PEG-precipitated bacteriophage particles.

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Acknowledgements

We thank Kathryn Cross (Imaging Platform, IFR) for microscopy, and K. I’Anson (IFR) and M. Brett for kind provision of strains. This research was funded by the Biotechnology and Biological Sciences Research Council (Grant BB/J004529/1), the EU-funded TORNADO grant (FP7-KBBE-222720) and the University of Messina.

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Authors and Affiliations

  1. Institute of Food Research, Colney, Norwich, NR4 7UA, UK

    Teresa Gervasi, Arjan Narbad & Melinda J. Mayer

  2. Dipartimento S. A. S. T. A. S., University of Messina, Viale F. Stagno d’Alcontres, Messina, Italy

    Teresa Gervasi & Rosario Lo Curto

Authors
  1. Teresa Gervasi
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  2. Rosario Lo Curto
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  3. Arjan Narbad
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  4. Melinda J. Mayer
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Corresponding author

Correspondence to Melinda J. Mayer.

Additional information

The complete genome sequence of ΦCP51 was deposited in the GenBank database under accession number KC237729.

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Gervasi, T., Curto, R.L., Narbad, A. et al. Complete genome sequence of ΦCP51, a temperate bacteriophage of Clostridium perfringens . Arch Virol 158, 2015–2017 (2013). https://doi.org/10.1007/s00705-013-1647-1

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  • DOI: https://doi.org/10.1007/s00705-013-1647-1

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