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Molecular characterisation of two divergent variants of grapevine leafroll-associated virus 3 in New Zealand

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Abstract

Partial genomic sequences of two divergent grapevine leafroll-associated virus 3 (GLRaV-3) variants, NZ1-B and NZ2, from New Zealand were determined and analysed (11,827 nt and 7,612 nt, respectively). At the nucleotide level, both variants are more than 20 % different from the previously published GLRaV-3 sequences, from phylogenetic groups 1 to 5. Phylogenetic analysis indicated that NZ1-B is a variant of the previously identified divergent NZ-1, while NZ2 is a novel sequence with only 76 % nucleotide sequence identity to GLRaV-3 variants NZ-1, GH11, and GH30. Therefore, NZ2 is a new variant of GLRaV-3. Amino acid sequence analysis of the NZ1-B and NZ2 coat proteins indicated significant substitutions that are predicted to alter the coat protein structure, which potentially leads to the observed reduced immunological reactivity of both variants to the Bioreba anti-GLRaV-3 conjugated monoclonal antibody.

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Acknowledgments

This research was supported by Corbans Viticulture Ltd, the University of Auckland, New Zealand Winegrowers, Plant and Food Research, and the Tertiary Education Commission.

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Authors and Affiliations

  1. School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand

    Kar Mun Chooi & Michael N. Pearson

  2. The New Zealand Institute for Plant and Food Research Limited, Private Bag 92169, Auckland, New Zealand

    Daniel Cohen

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  1. Kar Mun Chooi
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  2. Daniel Cohen
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  3. Michael N. Pearson
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Correspondence to Kar Mun Chooi.

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Chooi, K.M., Cohen, D. & Pearson, M.N. Molecular characterisation of two divergent variants of grapevine leafroll-associated virus 3 in New Zealand. Arch Virol 158, 1597–1602 (2013). https://doi.org/10.1007/s00705-013-1631-9

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  • DOI: https://doi.org/10.1007/s00705-013-1631-9

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