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Detection and partial molecular characterization of atypical plum pox virus isolates from naturally infected sour cherry

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Abstract

Atypical isolates of plum pox virus (PPV) were discovered in naturally infected sour cherry in urban ornamental plantings in Moscow, Russia. The isolates were detected by polyclonal double antibody sandwich ELISA and RT-PCR using universal primers specific for the 3′-non-coding and coat protein (CP) regions of the genome but failed to be recognized by triple antibody sandwich ELISA with the universal monoclonal antibody 5B and by RT-PCR using primers specific to for PPV strains D, M, C and W. Sequence analysis of the CP genes of nine isolates revealed 99.2-100 % within-group identity and 62-85 % identity to conventional PPV strains. Phylogenetic analysis showed that the atypical isolates represent a group that is distinct from the known PPV strains. Alignment of the N-terminal amino acid sequences of CP demonstrated their close similarity to those of a new tentative PPV strain, CR.

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Acknowledgments

We would like to thank Dr. Delano James for critical reading of the manuscript.

Conflict of interest

The authors declare no conflict of interests.

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Authors and Affiliations

  1. Department of Virology, Biological Faculty, Lomonosov Moscow State University, Leninskie Gory 1/12, Moscow, 119991, Russia

    Sergei Chirkov, Peter Ivanov & Anna Sheveleva

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  1. Sergei Chirkov
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  2. Peter Ivanov
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  3. Anna Sheveleva
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Corresponding author

Correspondence to Sergei Chirkov.

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Fig. S1 Symptoms observed in the plant infected with the PPV isolate Pul-DS (JPEG 1.96 MB)

705_2013_1630_MOESM2_ESM.tif

Fig. S2 (A, B, C) Analysis of the PPV isolates from sour cherry by IC-RT-PCR with 3′NCR-specific primers (A), P1/P2 primers (B) and PPV-CR-specific primers (C). PC – universal positive control (Agritest); nc1 and nc2 – negative controls (healthy cherry and Agritest, respectively). M – GeneRuler Plus 100 bp DNA ladder (Fermentas). There was no positive control in PCR using PPV-CR-specific primers (C) (TIFF 5250 kb)

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Chirkov, S., Ivanov, P. & Sheveleva, A. Detection and partial molecular characterization of atypical plum pox virus isolates from naturally infected sour cherry. Arch Virol 158, 1383–1387 (2013). https://doi.org/10.1007/s00705-013-1630-x

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  • DOI: https://doi.org/10.1007/s00705-013-1630-x

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