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Genetic diversity of subgroup 1 ilarviruses from eastern Australia

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Abstract

This is the first report of the genetic diversity within ilarvirus subgroup 1 from eastern Australia. It supports the separation of tobacco streak virus (TSV) strains from parthenium (Parthenium hysterophorus) and crownbeard (Verbescina encelioides) based on serology and host specificity. It has confirmed one previously described strain of TSV as a member of the species Strawberry necrotic shock virus and another as a new subgroup 1 ilarvirus, ageratum latent virus (AgLV), from Ageratum houstonianum. A multiplex RT-PCR showed that the genetically distinct strains of TSV and AgLV were commonly found in symptomless infections in virus-specific alternative weed hosts growing over a wide geographical range in eastern Australia. TSV has been one of the most damaging viruses in Australian oilseed and pulse crops in recent years, and this study has provided the taxonomic knowledge essential for the development of control programs for these viruses.

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Acknowledgments

We thank Dr. David Teakle for providing a collection of archived isolates, including isolates -835, -837, -840 and -1025 used in this study. Dr. Ben Callaghan and Dr. Paul Campbell provided advice to enable sequencing of complete genome ends. Dr. Alistair McTaggart assisted with phylogenetic analysis. Mr. Denis Persley assisted with collection of field samples. This study was jointly funded by the Grains Research Development Corporation project DAQ00130 and the Cotton Research and Development Corporation project DAQ0002.

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Authors and Affiliations

  1. Department of Agriculture, Fisheries and Forestry, Ecosciences Precinct, GPO Box 267, Brisbane, Queensland, 4001, Australia

    M. Sharman

  2. Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St. Lucia campus, Queensland, Australia

    M. Sharman

  3. Queensland Alliance for Agriculture and Food Innovation, The University of Queensland Ecosciences Precinct, GPO Box 267, Brisbane, Queensland, 4001, Australia

    J. E. Thomas

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  1. M. Sharman
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Correspondence to M. Sharman.

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Sharman, M., Thomas, J.E. Genetic diversity of subgroup 1 ilarviruses from eastern Australia. Arch Virol 158, 1637–1647 (2013). https://doi.org/10.1007/s00705-013-1628-4

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