Skip to main content
SpringerLink
Log in

The complete nucleotide sequence of sweet potato C6 virus: a carlavirus lacking a cysteine-rich protein

  • Annotated Sequence Record
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

The complete nucleotide sequence of a sweet potato virus, first identified two decades ago as virus “C-6”, was determined in this study. Sequence similarity and phylogenetic analysis clearly place it as a member of a distinct species within the genus Carlavirus, family Betaflexiviridae. Its genome structure was typical for that of other carlaviruses except that the ORF for the cysteine-rich protein was replaced by an ORF encoding a predicted protein with no similarity to any known protein.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  1. Fuentes S (1994) Preliminary identification of a sweet potato virus (C-6). Fitopatologia 29:38 (Abstract)

    Google Scholar 

  2. Fuentes S, Heider B, Tasso RC, Romero E, Zum Felde T, Kreuze JF (2012) Complete genome sequence of a potyvirus infecting yam beans (Pachyrhizus spp.) in Peru. Arch Virol 157(4):773–776. doi:10.1007/s00705-011-1214-6

    Article  PubMed  CAS  Google Scholar 

  3. Fuentes S, Salazar L (1989) Identification of sweet potato (Ipomoea batatas (L.) Lam.) viruses. Fitoptatologia 24:43 (Abstract)

    Google Scholar 

  4. Gramstat A, Prüfer D, Rohde W (1994) The nucleic acid-binding zinc finger protein of potato virus M is translated by internal initiation as well as by ribosomal frameshifting involving a shifty stop codon and a novel mechanism of P-site slippage. Nucleic Acids Res 22(19):3911–3917. doi:10.1093/nar/22.19.3911

    Article  PubMed  CAS  Google Scholar 

  5. King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (2012) Virus taxonomy: classification and nomenclature of viruses. Ninth Report of the International Committee on Taxonomy of Viruses. Elsevier Academic Press, San Diego

    Google Scholar 

  6. Loebenstein G, Thottappilly G, Fuentes S, Cohen J (2009) Virus and phytoplasma diseases. In: Loebenstein G, Thottappilly G (eds) The Sweetpotato, Part I. Springer Sciences Business Media BV, Dordrecht, pp 105–134. doi:10.1007/978-1-4020-9475-0_8

  7. Martelli GP, Adams MJ, Kreuze JF, Dolja VV (2007) Family Flexiviridae: a case study in virion and genome plasticity. Ann Rev Phytopathol 45:73–100. doi:10.1146/annurev.phyto.45.062806.094401

    Article  CAS  Google Scholar 

  8. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28(10):2731–2739. doi:10.1093/molbev/msr121

    Article  PubMed  CAS  Google Scholar 

  9. Turner R, Foster GD (1997) Deletion analysis of a translational enhancer upstream from the coat protein open reading frame of potato virus S. Arch Virol 142(1):167–175. doi:10.1007/s007050050067

    Article  PubMed  CAS  Google Scholar 

  10. Untiveros M, Fuentes S, Salazar LF (2007) Synergistic interaction of sweet potato chlorotic stunt virus (Crinivirus) with carla-, cucumo-, ipomo-, and potyviruses infecting sweet potato. Plant Dis 91(6):669–676

    Article  Google Scholar 

  11. Zerbino DR, Birney E (2008) Velvet: Algorithms for de novo short read assembly using de Bruijn graphs. Genome Res 18(5):821–829. doi:10.1101/gr.074492.107

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

We gratefully acknowledge the financial contribution from ICGEB-TWAS-UNESCO/IBSP Joint Programme on Capacity Building in Basic Molecular Biology and SLU 2011 funding for international collaboration. This work was also partially funded by the Bill & Melinda Gates Foundation through the Sweet potato Action for Security and Health in Africa project. The findings and conclusions contained within are those of the authors and do not necessarily reflect positions or policies of the Bill & Melinda Gates Foundation. We thank J. Arellano for technical assistance in maintaining the virus isolate in the greenhouse.

Author information

Author notes

  1. Wilmer Cuellar

    Present address: International Center for Tropical Agriculture (CIAT), Apartado Aéreo 6713, Cali, Colombia

Authors and Affiliations

  1. International Potato Center (CIP), Apartado 1558, Lima 12, Peru

    Joao De Souza, Segundo Fuentes, Wilmer Cuellar & Jan F. Kreuze

  2. Department of Plant Biology and Forest Genetics, Uppsala BioCenter SLU, PO Box 7080, 750 07, Uppsala, Sweden

    Eugene I. Savenkov

Authors
  1. Joao De Souza
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Segundo Fuentes
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Eugene I. Savenkov
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Wilmer Cuellar
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jan F. Kreuze
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jan F. Kreuze.

Rights and permissions

Reprints and permissions

About this article

Cite this article

De Souza, J., Fuentes, S., Savenkov, E.I. et al. The complete nucleotide sequence of sweet potato C6 virus: a carlavirus lacking a cysteine-rich protein. Arch Virol 158, 1393–1396 (2013). https://doi.org/10.1007/s00705-013-1614-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-013-1614-x

Keywords

Search

Navigation