Archives of Virology

, Volume 158, Issue 3, pp 695–699 | Cite as

Isolation of a novel herpesvirus from a Pacific white-sided dolphin

  • Keita Noguchi
  • Hiroshi Shimoda
  • Yutaka Terada
  • Masayuki Shimojima
  • Kaoru Kohyama
  • Yasuo Inoshima
  • Ken Maeda
Brief Report

Abstract

During establishment of primary cell culture from the kidney of a dead Pacific white-sided dolphin (Lagenorhynchus obliquidens), a cytopathic effect was observed. Polymerase chain reaction with a set of herpesvirus consensus primers yielded a fragment of the expected size. Nucleotide sequencing of the product indicated that the isolated virus was closely related to an alphaherpesvirus detected in a bottlenose dolphin in the United States, but the sequence identity at the protein level was low (86.6 %). Phylogenetic analysis of the encoded sequence confirmed that the new isolate belonged to the subfamily Alphaherpesvirinae and clustered together with other cetacean alphaherpesviruses. The complete gene encoding glycoprotein B (2,757 bp) was amplified from the novel isolate; the encoded protein was compared with the corresponding protein of other herpesviruses, revealing that this virus belongs to the genus Varicellovirus. Taken together, these results suggest that this virus corresponds to a novel herpesvirus capable of infecting Pacific white-sided dolphins.

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Copyright information

© Springer-Verlag Wien 2012

Authors and Affiliations

  • Keita Noguchi
    • 1
  • Hiroshi Shimoda
    • 1
  • Yutaka Terada
    • 1
  • Masayuki Shimojima
    • 1
  • Kaoru Kohyama
    • 2
  • Yasuo Inoshima
    • 3
  • Ken Maeda
    • 1
  1. 1.Laboratory of Veterinary Microbiology, Joint Faculty of Veterinary MedicineYamaguchi UniversityYamaguchiJapan
  2. 2.Izu-Mito Sea ParadiseNumazuJapan
  3. 3.Laboratory of Food and Environmental Hygiene, Department of Veterinary MedicineGifu UniversityGifuJapan

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