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Genomic characterization of echovirus 6 causing aseptic meningitis in Hokkaido, Japan: a novel cluster in the nonstructural protein coding region of human enterovirus B

Archives of Virology volume 158, pages 775–784 (2013)Cite this article

Abstract

We determined four complete nucleotide sequences of echovirus 6 (E6) isolated from an epidemic of aseptic meningitis (AM) in Hokkaido, Japan, in 2011. Phylogenetic analysis of the genes encoding viral capsid protein 1 revealed that the strains were closely related to E6 strains isolated in China in recent years, but they were distantly related to E6 strains isolated from patients with AM in Osaka Prefecture, Japan, in 2011. The genes encoding the viral protease and RNA-dependent RNA polymerase (3CD) were closely related to those of several non-E6 strains of the species Human enterovirus B isolated in China, South Korea, and Australia from 1999 to 2010, resulting in a novel cluster in the phylogenetic tree. These results suggest that the incidence of AM in Japan in 2011 was caused by at least two lineages of E6 strains, and a lineage of the 3CD gene was interspersed among different serotypic strains isolated in Western Pacific countries.

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Acknowledgments

The authors are grateful to the staff of the local hospital and of the regional Public Health Office, Naosuke Saji, Department of Health and Welfare, Hokkaido Government, for collecting samples and providing epidemiological information. This work was financially supported in part by the scientific grant from Hokkaido Government, Japan.

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Affiliations

  1. Center for Infectious Diseases Control, Hokkaido Institute of Public Health, North 19 West 12, Kita-ku, Sapporo, 060-0819, Japan

    Masahiro Miyoshi, Rika Komagome, Setsuko Ishida, Hideki Nagano, Kenichi Takahashi & Motohiko Okano

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  1. Masahiro Miyoshi
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  2. Rika Komagome
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  3. Setsuko Ishida
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  4. Hideki Nagano
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  6. Motohiko Okano
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Correspondence to Masahiro Miyoshi.

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Miyoshi, M., Komagome, R., Ishida, S. et al. Genomic characterization of echovirus 6 causing aseptic meningitis in Hokkaido, Japan: a novel cluster in the nonstructural protein coding region of human enterovirus B. Arch Virol 158, 775–784 (2013). https://doi.org/10.1007/s00705-012-1535-0

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  • DOI: https://doi.org/10.1007/s00705-012-1535-0

Keywords

  • Aseptic Meningitis
  • Complete Nucleotide Sequence
  • Nucleotide Sequence Identity
  • Nonstructural Protein
  • Acute Flaccid Paralysis