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Genetic diversity of porcine sapoviruses, kobuviruses, and astroviruses in asymptomatic pigs: an emerging new sapovirus GIII genotype

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Abstract

Small, non-enveloped RNA viruses belonging to the genera Sapovirus, Kobuvirus, and Mamastrovirus are usually associated with gastroenteritis in humans and animals. These enteric pathogens are considered potential zoonotic agents. In this study, the prevalence and genetic diversity of sapoviruses (SaVs), kobuviruses (KoVs), and astroviruses (AstVs) in asymptomatic pigs were investigated using a PCR approach. KoV was found to be the most prevalent virus (87.3 %), followed by AstV (34.2 %) and SaV (10.2 %). Interestingly, the intra- and inter-cluster distances between porcine SaV capsid sequences revealed one strain (P38/11/CZ) that formed a new genotype within genogroup III of porcine SaVs, and it is tentatively called “P38/11-like” genotype. Moreover, this is the first report of porcine kobuvirus detection on Czech pig farms. The high prevalence rate of gastroenteritis-producing viruses in clinically healthy pigs represents a continuous source of infection of pigs, and possibly to humans.

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Acknowledgments

We thank Jindriska Bendova and Andrea Kyjovska for excellent technical assistance. This work was supported by the Ministry of Agriculture of the Czech Republic (Grant No. NAZV QH81061) and the Ministry of Education, Youth and Sports of the Czech Republic (Grant No. AdmireVet CZ.1.05/2.1.00/01.006, ED006/01/01).

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The authors declare that they have no actual or potential conflict of interest.

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Correspondence to Lucie Dufkova.

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Nucleotide sequence data reported in this paper are available in the GenBank database under the accession numbers JX232612-JX232627.

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Dufkova, L., Scigalkova, I., Moutelikova, R. et al. Genetic diversity of porcine sapoviruses, kobuviruses, and astroviruses in asymptomatic pigs: an emerging new sapovirus GIII genotype. Arch Virol 158, 549–558 (2013). https://doi.org/10.1007/s00705-012-1528-z

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  • DOI: https://doi.org/10.1007/s00705-012-1528-z

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