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Molecular characterization of artemisia virus A, a new sobemovirus isolated from Artemisia annua

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Abstract

A distinct sobemovirus was isolated from diseased Artemisia annua plants grown in experimental culture plots in Switzerland. Electron microscopy performed on extracts of leaf and root samples of a diseased A. annua plant revealed icosahedral-30 nm viral particles. The complete nucleotide sequence of the viral genome was determined. The single positive-strand RNA of 4138 nt encodes four open reading frames with an organization similar to that described for sobemoviruses. Phylogenetic analysis revealed a close relationship to ryegrass mottle virus. The virus was efficiently acquired by healthy A. annua from contaminated soil samples. “Artemisia virus A” is tentatively proposed as a name for this new candidate member of the unassigned genus Sobemovirus.

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Acknowledgment

The technical assistance of N. Dubuis, J. Brodard, and P.-H. Crausaz is gratefully acknowledged. We kindly acknowledge Dr. P. Vinciguerra for critical reading of the manuscript.

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Author notes

  1. Carole Balmelli

    Present address: Service de la consommation et des affaires vétérinaires, 2000, Neuchâtel, Switzerland

Authors and Affiliations

  1. Agroscope Changins-Wädenswil Research Station ACW, 1260, Nyon, Switzerland

    Maria-Elena Ramel, Paul Gugerli & Carole Balmelli

  2. Service de l’agriculture, office d’arboriculture et cultures maraîchères, 1950, Sion, Switzerland

    Sébastien Besse

  3. Mediplant, centre de recherche de Conthey, 1964, Conthey, Switzerland

    Xavier Simonnet

Authors
  1. Maria-Elena Ramel
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  2. Paul Gugerli
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  3. Sébastien Besse
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  4. Xavier Simonnet
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  5. Carole Balmelli
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Correspondence to Carole Balmelli.

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Ramel, ME., Gugerli, P., Besse, S. et al. Molecular characterization of artemisia virus A, a new sobemovirus isolated from Artemisia annua . Arch Virol 158, 463–466 (2013). https://doi.org/10.1007/s00705-012-1507-4

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  • DOI: https://doi.org/10.1007/s00705-012-1507-4

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