Abstract
Classical swine fever (CSF) is a major constraint to pig production worldwide, and in many developing countries, the epidemiological status is unknown. Here, for the first time, molecular identification and characterization of CSFV isolates from two recent outbreaks in Nepal are presented. Analysis of full-length E2-encoding sequences revealed that these isolates belonged to CSFV subgenotype 2.2 and had highest genetic similarity to isolates from India. Hence, for CSFV, Nepal and India should be regarded as one epidemiological unit. Both Nepalese isolates exhibited significant sequence differences, excluding a direct epidemiological connection and suggesting that CSFV is endemic in that country.
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Acknowledgments
This study was part of the activities of the EU and OIE Reference Laboratory for Classical Swine Fever and supported by DG SANCO of the European Commission. We are grateful to Gabriele Müller, Benjamin Ostermann, and Karin Ruthenberg (EURL, Hannover, Germany) for excellent technical assistance. We thank V.K. Karn (Regional Veterinary Laboratory, Janakpur, Nepal) for providing sample material from the Makwanpur district of Nepal.
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Postel, A., Jha, V.C., Schmeiser, S. et al. First molecular identification and characterization of classical swine fever virus isolates from Nepal. Arch Virol 158, 207–210 (2013). https://doi.org/10.1007/s00705-012-1463-z
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DOI: https://doi.org/10.1007/s00705-012-1463-z