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Bacteriophage vB_EcoM_FV3: a new member of “rV5-like viruses”

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Abstract

A proposed new genus of the family Myoviridae, “rV5-like viruses”, includes two lytic bacteriophages: Escherichia coli O157: H7-specific bacteriophage rV5 and Salmonella phage PVP-SE1. Here, we present basic properties and genomic characterization of a novel rV5-like phage, vB_EcoM_FV3, which infects E. coli K-12-derived laboratory strains and replicates at high temperature (up to 47 °C). The 136,947-bp genome of vB_EcoM_FV3 contains 218 open reading frames and encodes 5 tRNAs. The genomic content and organization of vB_EcoM_FV3 is more similar to that of rV5 than to PVP-SE1, but all three phages share similar morphological characteristics and form a homogeneous phage group.

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Acknowledgments

We thank Dr. Ken Kreuzer, Dr. Victor Krylov and Dr. Lindsay W. Black for E. coli strains. This work was supported in part by the Lithuanian State Science and Studies Foundation (Grant No. N-07005) and was also funded by a grant (No. MIP-76/2010) from the Research Council of Lithuania.

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Authors and Affiliations

  1. Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry of Vilnius University, Mokslininkų 12, 08662, Vilnius, Lithuania

    Lidija Truncaite, Eugenijus Šimoliūnas, Aurelija Zajančkauskaite, Laura Kaliniene, Roma Mankevičiūte, Vytautas Klausa & Rolandas Meškys

  2. Laboratory of Plant Viruses, Institute of Botany of the Nature Research Centre, Žaliųjų Ežerų 49, 08406, Vilnius, Lithuania

    Juozas Staniulis

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  1. Lidija Truncaite
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  2. Eugenijus Šimoliūnas
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Correspondence to Lidija Truncaite.

Electronic supplementary material

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705_2012_1449_MOESM1_ESM.doc

Supplementary material 1 Table 1S. A list of bacteriophage vB_EcoM_FV3 putative genes with predicted functions. (DOC 285 kb)

705_2012_1449_MOESM2_ESM.tif

Supplementary material 2 Fig. 1S. Functional genomic map of bacteriophage vB_EcoM_FV3. Putative genes with predicted coding functions are represented as arrows. The colour code for the online version of the article is as follows: yellow—DNA replication, recombination, repair and packaging; brown—transcription, translation, nucleotide metabolism; blue—virion structure; green—chaperones/assembly; purple—lysis, host or phage interactions; grey—ORFs of unknown function; red—unique FV3 ORFs of unknown function; black—tRNA. (TIFF 4748 kb)

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Truncaite, L., Šimoliūnas, E., Zajančkauskaite, A. et al. Bacteriophage vB_EcoM_FV3: a new member of “rV5-like viruses”. Arch Virol 157, 2431–2435 (2012). https://doi.org/10.1007/s00705-012-1449-x

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  • DOI: https://doi.org/10.1007/s00705-012-1449-x

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