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Detection and molecular characterization of hepatitis E virus in clinical, environmental and putative animal sources

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Abstract

Putative animal reservoirs and environmental samples were studied to investigate potential routes of transmission for indigenous hepatitis E virus (HEV) infection in Hokkaido, Japan. A total of 468 liver samples and 954 environmental samples were collected from 2003 to 2011 for this study. Four swine livers (1 %) were positive for HEV RNA; two strains belonged to genotype 3 and the other two strains were genotype 4. Genotype 3 HEV was detected in a sewage sample and a seawater sample. HEV strains derived from swine liver, seawater and raw sewage samples shared 93-100 % sequence similarity with human HEV strains.

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Acknowledgments

This study was supported by grant 21590725 for Scientific Research (C) from the Ministry of Education, Culture, Sports, Science and Technology of Japan. We are grateful to Shinichi Kudo for advice and encouragement during this study.

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Authors and Affiliations

  1. Department of Infectious Diseases, Hokkaido Institute of Public Health, North 19, West 12, Kita-ku, Sapporo, 060-0819, Japan

    Setsuko Ishida, Shima Yoshizumi, Tetsuya Ikeda, Masahiro Miyoshi & Akiko Goto

  2. Japanese Red Cross Hokkaido Block Blood Center, Sapporo, Japan

    Keiji Matsubayashi & Hisami Ikeda

Authors
  1. Setsuko Ishida
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  2. Shima Yoshizumi
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  3. Tetsuya Ikeda
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  4. Masahiro Miyoshi
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  6. Keiji Matsubayashi
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  7. Hisami Ikeda
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Correspondence to Setsuko Ishida.

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Ishida, S., Yoshizumi, S., Ikeda, T. et al. Detection and molecular characterization of hepatitis E virus in clinical, environmental and putative animal sources. Arch Virol 157, 2363–2368 (2012). https://doi.org/10.1007/s00705-012-1422-8

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  • DOI: https://doi.org/10.1007/s00705-012-1422-8

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