Skip to main content
SpringerLink
Log in

The pan-genotype specificity of the hepatitis C virus anti-core monoclonal antibody C7-50 is contingent on the quasispecies profile of a population

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

The inter/intra-genotype quasispecies makeup of hepatitis C virus (HCV) has retarded the development of antibodies capable of pan-genotype reactivity. Mutations, even in conserved domains, are tolerated to a degree. In this report, we characterise the pan-genotype specificity of the commercially available monoclonal anti-HCV core antibody C7-50. We demonstrate the antibody’s ability to detect HCV core protein following infection of Huh7 cells with serum-derived HCV of genotypes 2-5 and that a single-site polymorphism in a genotype 3a core amino acid sequence is sufficient to disrupt antibody recognition of the epitope. This same polymorphism is a feature of genotype 3 viruses.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

References

  1. Akazawa D, Date T, Morikawa K, Murayama A, Miyamoto M, Kaga M, Barth H, Baumert TF, Dubuisson J, Wakita T (2007) CD81 expression is important for the permissiveness of Huh7 cell clones for heterogeneous hepatitis C virus infection. J Virol 81:5036–5045

    Article  PubMed  CAS  Google Scholar 

  2. Bartenschlager R, Lohmann V (2000) Replication of hepatitis C virus. J Gen Virol 81:1631–1648

    PubMed  CAS  Google Scholar 

  3. Bartenschlager R, Pietschmann T (2005) Efficient hepatitis C virus cell culture system: what a difference the host cell makes. Proc Natl Acad Sci USA 102:9739–9740

    Article  PubMed  CAS  Google Scholar 

  4. Bensadoun P, Rodriguez C, Soulier A, Higgs M, Chevaliez S, Pawlotsky JM (2011) Genetic background of hepatocyte cell lines: Are in vitro hepatitis C virus research data reliable? Hepatology 54:748

    Article  PubMed  Google Scholar 

  5. Boulant S, Montserret R, Hope RG, Ratinier M, Targett-Adams P, Lavergne JP, Penin F, McLauchlan J (2006) Structural determinants that target the hepatitis C virus core protein to lipid droplets. J Biol Chem 281:22236–22247

    Article  PubMed  CAS  Google Scholar 

  6. Buck M (2008) Direct infection and replication of naturally occurring hepatitis C virus genotypes 1, 2, 3 and 4 in normal human hepatocyte cultures. PLoS One 3:e2660

    Article  PubMed  Google Scholar 

  7. Crooks GE, Hon G, Chandonia JM, Brenner SE (2004) WebLogo: a sequence logo generator. Genome Res 14:1188–1190

    Article  PubMed  CAS  Google Scholar 

  8. Duffy S, Shackelton LA, Holmes EC (2008) Rates of evolutionary change in viruses: patterns and determinants. Nat Rev Genet 9:267–276

    Article  PubMed  CAS  Google Scholar 

  9. Durantel D, Zoulim F (2007) Going towards more relevant cell culture models to study the in vitro replication of serum-derived hepatitis C virus and virus/host cell interactions? J Hepatol 46:1–5

    Article  PubMed  CAS  Google Scholar 

  10. Esteban JI, Sauleda S, Quer J (2008) The changing epidemiology of hepatitis C virus infection in Europe. J Hepatol 48:148–162

    Article  PubMed  Google Scholar 

  11. Kalinina O, Norder H, Mukomolov S, Magnius LO (2002) A natural intergenotypic recombinant of hepatitis C virus identified in St. Petersburg. J Virol 76:4034–4043

    Article  PubMed  CAS  Google Scholar 

  12. Kuiken C, Yusim K, Boykin L, Richardson R (2005) The Los Alamos hepatitis C sequence database. Bioinformatics 21:379–384

    Article  PubMed  CAS  Google Scholar 

  13. Li H, Thomassen LV, Majid A, McMahon BJ, Bruden D, McArdle S, Bano N, Chung M, Carithers RL, Perkins JD, Sullivan DG, Gretch DR (2008) Investigation of putative multisubtype hepatitis C virus infections in vivo by heteroduplex mobility analysis of core/envelope subgenomes. J Virol 82:7524–7532

    Article  PubMed  CAS  Google Scholar 

  14. Miyanari Y, Atsuzawa K, Usuda N, Watashi K, Hishiki T, Zayas M, Bartenschlager R, Wakita T, Hijikata M, Shimotohno K (2007) The lipid droplet is an important organelle for hepatitis C virus production. Nat Cell Biol 9:1089–1097

    Article  PubMed  CAS  Google Scholar 

  15. Moreau I, Hegarty S, Levis J, Sheehy P, Crosbie O, Kenny-Walsh E, Fanning LJ (2006) Serendipitous identification of natural intergenotypic recombinants of hepatitis C in Ireland. Virol J 3:95

    Article  PubMed  Google Scholar 

  16. Muerhoff AS, Gutierrez R, Kyrk C, Leary T, Schlauder G, Dawson G, Desai SM (2008) Genotype dependence of peptide-based immunoassays for the detection of HCV core antibodies. J Med Virol 80:411–418

    Article  PubMed  CAS  Google Scholar 

  17. Nadarajah R, Khan GY, Miller SA, Brooks GF (2007) Evaluation of a new-generation line-probe assay that detects 5 ‘ untranslated and core regions to genotype and subtype hepatitis C virus. Am J Clin Pathol 128:300–304

    Article  PubMed  Google Scholar 

  18. Sainz B Jr, Barretto N, Uprichard SL (2009) Hepatitis C virus infection in phenotypically distinct Huh7 cell lines. PLoS One 4:e6561

    Article  PubMed  Google Scholar 

  19. Sainz B Jr, Barretto N, Yu X, Corcoran P, Uprichard SL (2012) Permissiveness of human hepatoma cell lines for HCV infection. Virol J 9:30

    Article  PubMed  CAS  Google Scholar 

  20. Seipp S, Mueller HM, Pfaff E, Stremmel W, Theilmann L, Goeser T (1997) Establishment of persistent hepatitis C virus infection and replication in vitro. J Gen Virol 78(Pt 10):2467–2476

    PubMed  CAS  Google Scholar 

  21. Simmonds P (2004) Genetic diversity and evolution of hepatitis C virus–15 years on. J Gen Virol 85:3173–3188

    Article  PubMed  CAS  Google Scholar 

  22. Tuke PW, Grant PR, Waite J, Kitchen AD, Eglin RP, Tedder RS (2008) Hepatitis C virus window-phase infections: closing the window on hepatitis C virus. Transfusion 48:594–600

    Article  PubMed  Google Scholar 

  23. Wang XH, Netski DM, Astemborski J, Mehta SH, Torbenson MS, Thomas DL, Ray SC (2007) Progression of fibrosis during chronic hepatitis C is associated with rapid virus evolution. J Virol 81:6513–6522

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Molecular Virology Diagnostic and Research Laboratory, Department of Medicine, University College Cork, Clinical Sciences Building, Cork, Ireland

    Brendan A. Palmer, John Menton, John Levis & Liam J. Fanning

  2. Department of Gastroenterology, Cork University Hospital, Cork, Ireland

    Elizabeth Kenny-Walsh & Orla Crosbie

Authors
  1. Brendan A. Palmer
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. John Menton
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. John Levis
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Elizabeth Kenny-Walsh
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Orla Crosbie
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Liam J. Fanning
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Liam J. Fanning.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Palmer, B.A., Menton, J., Levis, J. et al. The pan-genotype specificity of the hepatitis C virus anti-core monoclonal antibody C7-50 is contingent on the quasispecies profile of a population. Arch Virol 157, 2235–2239 (2012). https://doi.org/10.1007/s00705-012-1418-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-012-1418-4

Keywords

Search

Navigation