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First complete and productive cell culture model for members of the genus Iridovirus

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Abstract

Chilo iridescent virus (CIV; the type strain of the genus Iridovirus) replicates productively in larvae of the boll weevil, Anthonomus grandis. This study focuses on characterizing productive infections of a boll weevil cell line, BRL-AG-3A (AG3A), starting with CIV reared in the waxworm, Galleria mellonella. We show that CIV can be continually and productively passaged to high titer in AG3A cells. The replication of larval-derived CIV in AG3A was analyzed by observing viral DNA replication and restriction endonuclease digestion profiles, morphogenesis, and infectivity using TCID50 assays with AG3A as an indicator cell line. The data showed that virus passaged in the AG3A host is stable. AG3A cells are more efficient than previously utilized CF-124T cells from Choristoneura fumiferana. This system constitutes a superior model for cellular and molecular studies on CIV; it represents the first complete, productive cell culture model for the replication of CIV or any member of the genus Iridovirus.

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Acknowledgments

This work was supported in part by grants to S. L. B. from Texas Advanced Research Program Grant No. 0036444-0148, Texas Advanced Technology Program Grant No. 0036444-0108, and Texas Advanced Technology Program Grant No. 0036444-0046. Support was also provided by Office of the Vice-President for Research, Office of the Provost, and Institute for Biotechnology at Texas Tech University. S. M. D. was partially supported by Summer Research Awards from the Graduate School and the Department of Biological Sciences, Texas Tech University. We are very grateful to Dr. James Kalmakoff for the generous gift of iridescent virus samples and to Dr. Arthur MacIntosh for the gift of BRL-AG-3A cells. We thank Drs. Candace Haigler and Mark Grimson for use of electron microscopy facilities, and Saranya Ganapathy for technical assistance.

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Authors and Affiliations

  1. Department of Biological Sciences, Texas Tech University, Box 43131, Lubbock, TX, 79409-3131, USA

    Susan M. D’Costa, David J. Vigerust, Marsha R. Perales-Hull, Sundus A. Lodhi, Polrit Viravathana & Shän L. Bilimoria

  2. The Center for Biotechnology and Genomics, Texas Tech University, Lubbock, TX, 79409-3131, USA

    Shän L. Bilimoria

  3. Department of Molecular Genetics and Microbiology, College of Medicine, University of Florida, P.O. Box 100266, JHMHC, Gainesville, FL, 32610, USA

    Susan M. D’Costa

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  1. Susan M. D’Costa
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  2. David J. Vigerust
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  3. Marsha R. Perales-Hull
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  4. Sundus A. Lodhi
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  5. Polrit Viravathana
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  6. Shän L. Bilimoria
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Correspondence to Shän L. Bilimoria.

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D’Costa, S.M., Vigerust, D.J., Perales-Hull, M.R. et al. First complete and productive cell culture model for members of the genus Iridovirus . Arch Virol 157, 2171–2178 (2012). https://doi.org/10.1007/s00705-012-1417-5

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  • DOI: https://doi.org/10.1007/s00705-012-1417-5

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