Abstract
Avian influenza viruses A/duck/Mongolia/47/2001 (H7N1) (47/01) and A/duck/Mongolia/867/2002 (H7N1) (867/02) were defined as low-pathogenic avian influenza viruses (LPAIVs) using an intravenous pathogenicity test in chickens. On the other hand, the intracerebral pathogenicity indices of 47/01 and 867/02 were 1.30 and 0.00, respectively. A series of reassortant viruses were generated between 47/01 and 867/02, and their intracerebral pathogenicity was compared in one-day-old chicks to identify the protein(s) responsible for the intracerebral pathogenicity of 47/01. The results indicate that the amino acids at positions 50 and 98 of the nucleoprotein are related to the pathogenicity of 47/01 in chicks by intracerebral inoculation. A significant association was found between mortality of the chicks inoculated intracerebrally with 47/01 and virus replication in the lungs and/or brain. These results indicate that the NP of avian influenza viruses may be responsible for intracerebral pathogenicity in the host.
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Acknowledgments
We are grateful to Dr. R. G. Webster, Dr. E. Hoffmann, and Dr. R. Webby, St. Jude Children’s Research Hospital, for kindly providing pHW2000 and pHW72-EGFP plasmids. The present work was supported in part by the Program of Founding Research Centers for Emerging and Reemerging Infectious Disease from the Ministry of Education, Culture, Sports, Science, and Technology, Japan, Solution-Oriented Research for Science and Technology (SORST) from the Japan Science and Technology Agency (JST), and Japan Racing and Livestock Promotion Foundation. We are thankful to Dr. K. Soda for providing kind suggestions regarding this manuscript.
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Isoda, N., Tsuda, Y., Asakura, S. et al. The nucleoprotein is responsible for intracerebral pathogenicity of A/duck/Mongolia/47/2001 (H7N1) in chicks. Arch Virol 157, 2257–2264 (2012). https://doi.org/10.1007/s00705-012-1415-7
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DOI: https://doi.org/10.1007/s00705-012-1415-7