Advertisement

Archives of Virology

, Volume 157, Issue 10, pp 1949–1957 | Cite as

Intraviral protein interactions of Chandipura virus

  • Kapila Kumar
  • Jyoti Rana
  • R. Sreejith
  • Reema Gabrani
  • Sanjeev K. Sharma
  • Amita Gupta
  • Vijay K. Chaudhary
  • Sanjay Gupta
Original Article

Abstract

Chandipura virus (CHPV) is an emerging rhabdovirus responsible for several outbreaks of fatal encephalitis among children in India. The characteristic structure of the virus is a result of extensive and specific interplay among its five encoded proteins. The revelation of interactions among CHPV proteins can help in gaining insight into viral architecture and pathogenesis. In the current study, we carried out comprehensive yeast two-hybrid (Y2H) analysis to elucidate intraviral protein-protein interactions. All of the interactions identified by Y2H were assessed for reliability by GST pull-down and ELISA. A total of eight interactions were identified among four viral proteins. Five of these interactions are being reported for the first time for CHPV. Among these, the glycoprotein (G)-nucleocapsid (N) interaction could be considered novel, as this has not been reported for any members of the family Rhabdoviridae. This study provides a framework within which the roles of the identified protein interactions can be explored further for understanding the biology of this virus at the molecular level.

Keywords

Rabies Virus Vesicular Stomatitis Virus Viral Life Cycle GAL4 Activation Domain pGADT7 Vector 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgment

This work was funded by research grant from Department of Science and Technology, Government of India.

References

  1. 1.
    Banerjee AK (1987) Transcription and replication of rhabdoviruses. Microbiol Rec 51:66–87Google Scholar
  2. 2.
    Barge A, Gaudin Y, Coulon P, Ruigrok RWH (1993) Vesicular Stomatitis virus M protein may be inside the ribonucleocapsid coil. J Virol 67(12):7246–7253PubMedGoogle Scholar
  3. 3.
    Basak S, Mondal A, Polley S, Mukhopadhyay S, Chattopadhyay D (2007) Reviewing Chandipura: a vesiculovirus in human epidemics. Biosci Rep 27(4):275–298PubMedCrossRefGoogle Scholar
  4. 4.
    Bhatt PN, Rodrigues FM (1967) Chandipura: a new Arbovirus isolated in India from patients with febrile illness. Indian J Med Res 55:1295–1305PubMedGoogle Scholar
  5. 5.
    Bieniasz PD (2006) Late budding domains and host proteins in enveloped virus release. Virol 344:55–63CrossRefGoogle Scholar
  6. 6.
    Calderwood MA, Venkatesan K, Xing L et al (2007) Epstein-Barr virus and virus human protein interaction maps. Proc Natl Acad Sci USA 104:7606–7611PubMedCrossRefGoogle Scholar
  7. 7.
    Chadha MS, Arankalle VA, Jadi RS, Joshi MV, Thakare JP (2005) An outbreak of Chandipura virus encephalitis in the eastern districts of Gujarat State; India. Am J Trop Med Hyg 73:566–570PubMedGoogle Scholar
  8. 8.
    Chattopadhyay D, Raha T, Chattopadhyay D (1997) Single serine phosphorylation within the acidic domain of Chandipura Virus protein regulates the transcription in vitro. Virol 239:11–19CrossRefGoogle Scholar
  9. 9.
    Chen M, Ogino T, Banerjee AK (2006) Mapping and functional role of the self-association domain of vesicular stomatitis virus phosphoprotein. J Virol 80(19):9511–9518PubMedCrossRefGoogle Scholar
  10. 10.
    Chen M, OginoT Banerjee AK (2007) Interaction of Vesicular Stomatitis virus P and N proteins: Identification of two overlapping domains at the N terminus of P that are involved in N0-P complex cormation and encapsidation of viral genome RNA. J Virol 81(24):13478–13485PubMedCrossRefGoogle Scholar
  11. 11.
    Chong LD, Rose JK (1993) Membrane association of functional Vesicular Stomatitis virus matrix protein in vivo. J Virol 6(1):407–441Google Scholar
  12. 12.
    Chong LD, Rose JK (1994) Interactions of normal and mutant Vesicular Stomatitis Virus matrix proteins with the plasma membrane and nucleocapsid. J Virol 68(1):441–447PubMedGoogle Scholar
  13. 13.
    D’agostino PM, Amenta JJ, Relss CS (2009) IFN- β-induces alteration of VSV protein phosphorylation in neuronal cells. Virol Immuno 22:353–369CrossRefGoogle Scholar
  14. 14.
    Dragunova J, Zavada J (1979) Cross-neutralisation between Vesicular Stomatitis virus type Indiana and Chandipura virus. Acta Virol 23:319–328PubMedGoogle Scholar
  15. 15.
    Fields S, Song O (1989) A novel genetic system to detect protein protein interactions. Nature 340:245–246PubMedCrossRefGoogle Scholar
  16. 16.
    Fossum E, Friedel CC, Rajagopala SV et al (2009) Evolutionarily conserved Herpes viral protein interaction networks. PLoS Path 5:e1000570CrossRefGoogle Scholar
  17. 17.
    Gao Y, Lenard J (1995) Multimerization and transcriptional activation of the phosphoprotein (P) of Vesicular Stomatitis virus by casein kinase-II. EMBO J 14(6):1240–1247PubMedGoogle Scholar
  18. 18.
    Ge P, Tsao J, Schein S, Green TJ, Luo M, Zhou ZH (2010) CryoEM model of the bullet-shaped vesicular stomatitis virus. Science 327(5966):689–693PubMedCrossRefGoogle Scholar
  19. 19.
    Graham SC, Assenberg A, Delmas O, Verma A, Gholami A, Talbi C, Owens RJ, Stuart DI, Grimes JM, Bourhy H (2008) Rhabdovirus matrix protein structures reveal a novel mode of self-association. PloS Path 4(12):e1000251Google Scholar
  20. 20.
    Gupta A (2009) Killing activity and rescue function of genome wide toxin-antitoxin loci of Mycobacterium Tuberculosis. FEMS Microbiol lett 290(1):45–53PubMedCrossRefGoogle Scholar
  21. 21.
    Harty RN, Paragas J, Sudol M, Palese P (1999) A proline rich motif within the matrix protein of Vesicular Stomatitis virus and Rabies virus interacts with WW domains of cellular proteins: implications for viral budding. J Virol 73:2921–2929PubMedGoogle Scholar
  22. 22.
    Hepojoki J, Strandin T, Wang H, Vapalathi O, Vaheri A, Lankinen H (2010) Cytoplasmic tails of Hantavirus glycoproteins interact with the nucleocapsid protein. J Gen Virol 91:2341–2350PubMedCrossRefGoogle Scholar
  23. 23.
    Ito TK, Muta TS, Ozawa R, Chiba T, Nishizawa M, Yamamoto K, Kuhara K, Sakaki Y (2000) Toward a protein-protein interaction map of the budding yeast: a comprehensive system to examine two-hybrid interactions in all possible combinations between the yeast proteins. Proc Natl Acad Sci USA 97:1143–1147PubMedCrossRefGoogle Scholar
  24. 24.
    Kumar K, Rana J, Guleria A, Gupta A, Chaudhary VK, Gupta S (2011) Expression and characterization of Chandipura Virus proteins. Res Biotech 2(6):27–36Google Scholar
  25. 25.
    Lyles DS, McKenzie M, Parce JW (1992) Subunit interactions of Vesicular Stomatitis virus envelope glycoprotein stabilized by binding to viral matrix protein. J Virol 66(1):349–358PubMedGoogle Scholar
  26. 26.
    Majumdar A, Bhattacharya R, Basak S, Shaila MS, Chattopadhyay D, Roy S (2004) P-protein of Chandipura virus is an N-protein-specific chaperone that acts at the nucleation stage. Biochem 43:2863–2870CrossRefGoogle Scholar
  27. 27.
    Majumder A, Basak S, Raha T, Chowdhury SP, Chattopadhyay D, Roy S (2001) Effect of osmolytes and chaperone-like action of P-protein on folding of nucleocapsid protein of Chandipura virus. J Biol Chem 276:30948–30955PubMedCrossRefGoogle Scholar
  28. 28.
    McCraith S, Holtzman T, Moss B, Fields S (2000) Genome wide analysis of Vaccinia virus protein protein interactions. Proc Natl Acad Sci USA 97:4879–4884PubMedCrossRefGoogle Scholar
  29. 29.
    Mebatsion T, Weiland F, Conzelmann KK (1999) Matrix protein of rabies virus is responsible for the assembly and budding of bullet-shaped particles and interacts with the transmembrane spike glycoprotein. J Gen Virol 73:242–250Google Scholar
  30. 30.
    Mondal A, Bhattacharya R, Ganguly T, Mukhopadhyay S, Basu A, Basak S, Chattopadhyay D (2010) Elucidation of functional domains of Chandipura virus nucleocapsid protein involved in oligomerization and RNA binding: implication in viral genome encapsidation. Virol 407(1):33–42CrossRefGoogle Scholar
  31. 31.
    Mudd JA, Swanson RE (1978) In situ cross-linking of Vesicular Stomatitis virus proteins with reversible agents. Virol 88:263–280CrossRefGoogle Scholar
  32. 32.
    Pulmanausahakul R, Li J, Schnell MJ, Dietzschold B (2008) The glycoprotein and the matrix protein of Rabies virus affect pathogenicity by regulating viral replication and facilitating cell-to-cell spread. J Virol 82:2330–2338PubMedCrossRefGoogle Scholar
  33. 33.
    Raha T, Samal E, Majumdar A, Basak S, Chattopadhyay D, Chattopadhyay DJ (2000) N-terminal region of P protein of Chandipura virus is responsible for phosphorylation-mediated homodimerization. Prot Eng 13(6):437–444CrossRefGoogle Scholar
  34. 34.
    Rao BL, Basu A, Wairagkar NS, Gore MM, Arankalle VA (2004) A large outbreak of acute encephalitis with high case fatality rate in children in Andhra Pradesh, India in 2003 associated with Chandipura virus. Lancet 364:869–874PubMedCrossRefGoogle Scholar
  35. 35.
    Roche S, Rey FA, Gaudin Y, Bressanelli S (2007) Structure of the prefusion form of the vesicular stomatitis virus glycoprotein G. Science 315(5813):843–848PubMedCrossRefGoogle Scholar
  36. 36.
    Rozen R, Sathish N, Li Y, Yuan Y (2008) Virion wide protein interactions of Kaposi’s Sarcoma associated Herpesvirus. J Virol 82(10):4742–4750PubMedCrossRefGoogle Scholar
  37. 37.
    Tandale BV, Tikute SS, Arankalle VA, Sathe PS, Joshi MV (2008) Chandipura virus: a major cause of acute encephalitis in children in North Telangana, Andhra Pradesh, India. J Med Virol 80:118–124PubMedCrossRefGoogle Scholar
  38. 38.
    Tao H, Liu W, Simmons BN, Harris HK, Cox TC, Massiah MA (2010) Purifying natively folded proteins from inclusion bodies using sarkosyl, Triton X-100 and CHAPS. Biotechniques 48(1):61–64PubMedCrossRefGoogle Scholar
  39. 39.
    Thomas D, Newcomb WW, Brown JC, Wall JS, Hainfeld JF, Trus BL, Steven AC (1985) Mass and molecular composition of Vesicular Stomatitis virus: a scanning transmission electron microscopy analysis. J Virol 54:598–607PubMedGoogle Scholar
  40. 40.
    Uetz P, Dong YA, Zeretzke C et al (2006) Herpes viral protein networks and their interaction with the human proteome. Science 311:239–242PubMedCrossRefGoogle Scholar
  41. 41.
    Walhout AJ, Vidal M (2001) High-throughput yeast two-hybrid assays for large-scale protein interaction mapping. Methods 24:297–306PubMedCrossRefGoogle Scholar
  42. 42.
    Zakowski JJ, Wagner RR (1980) Localization of membrane-associated proteins in vesicular stomatitis virus by the use of hydrophobic membrane probes and cross-linking reagents. J Virol 36:93–102PubMedGoogle Scholar

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Kapila Kumar
    • 1
  • Jyoti Rana
    • 1
  • R. Sreejith
    • 1
  • Reema Gabrani
    • 1
  • Sanjeev K. Sharma
    • 1
  • Amita Gupta
    • 2
  • Vijay K. Chaudhary
    • 3
  • Sanjay Gupta
    • 1
  1. 1.Department of Biotechnology, Centre for Emerging DiseasesJaypee Institute of Information TechnologyNoidaIndia
  2. 2.Department of MicrobiologyUniversity of Delhi South CampusNew DelhiIndia
  3. 3.Department of BiochemistryUniversity of Delhi South CampusNew DelhiIndia

Personalised recommendations