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Tobacco rattle virus genome alterations in the Hosta hybrid ‘Green Fountain’ and other plants: reassortments, recombinations and deletions

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Abstract

Tobacco rattle virus from a Hosta hybrid contained one RNA1 (Ho-1) and two RNA2 species (Ho-2a, Ho-2b). Whereas Ho-1 resembles TRV Al RNA1 from Alstroemerias, Ho-2a and Ho-2b resemble TRV TpO1 RNA2 from a potato field. Ho-2a has a complete RNA2-specific sequence, whereas that of Ho2-b carries a large deletion. The short RNA1-related 3’ end of Ho-2a is distinct from that of Ho-1, whereas the longer one of Ho-2b is identical to that of Ho-1. TRV RNA2 molecules may apparently become associated with different TRV RNA1 molecules, from which they can acquire 3’ends of various lengths while often losing large portions of their RNA2-specific sequences.

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Authors and Affiliations

  1. c/o Julius Kühn Institute, Federal Research Center for Cultivated Plants, Institute for Epidemiology and Pathogen Diagnostics, Messeweg 11, D38104, Braunschweig, Germany

    R. Koenig & D.-E. Lesemann

  2. Leiden Institute of Chemistry, Leiden University, P.O. Box 9502, 2300 RA, Leiden, The Netherlands

    C. W. A. Pleij

Authors
  1. R. Koenig
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  2. D.-E. Lesemann
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  3. C. W. A. Pleij
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Corresponding author

Correspondence to R. Koenig.

Additional information

The GenBank accession numbers for TRV Ho-1, Ho-2a and Ho-2b are JQ235203, JQ235204 and JQ235205, respectively. The sequences lack ca. 20 primer-derived nucleotides at their 5’ and 3’ ends.

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Koenig, R., Lesemann, DE. & Pleij, C.W.A. Tobacco rattle virus genome alterations in the Hosta hybrid ‘Green Fountain’ and other plants: reassortments, recombinations and deletions. Arch Virol 157, 2005–2008 (2012). https://doi.org/10.1007/s00705-012-1365-0

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  • DOI: https://doi.org/10.1007/s00705-012-1365-0

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