Skip to main content
SpringerLink
Log in

Tomato dwarf leaf virus, a New World begomovirus infecting tomato in Argentina

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

Begomovirus infection is becoming a threat in fresh-market tomato in Argentina, where mixed infections with begomoviruses are common. The complete sequence of a begomovirus isolate infecting tomato sampled in Salta was molecularly characterized. Phylogenetic analysis showed that this virus isolate is closely related to previously reported Brazilian, Bolivian and Argentinean begomoviruses. The associated symptoms in Nicotiana benthamiana and Solanum lycopersicum were determined by biolistic delivery of infectious DNA-A and DNA-B clones. This begomovirus isolate induced leaf mottling, rugosity and dwarfing, and growth retardation in tomato. Based on these symptoms, we propose the name of tomato dwarf leaf virus (ToDLV) for this new begomovirus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

References

  1. Stanley J, Bisaro DM, Briddon RW, Brown JK, Fauquet CM, Harrison BD, Rybicki EP, Stenger DC (2005) Geminiviridae. In: Fauquet CM MM, Maniloff J, Desselberger U, Ball LA (eds) Virus taxonomy, VIIIth report of the ICTV, Elsevier/Academic Press, London, pp 301–326

  2. Varma A, Malathi VG (2003) Emerging geminivirus problems: a serious threat to crop production. Ann Appl Biol 142:145–164

    Article  CAS  Google Scholar 

  3. Morales FJ, Anderson PK (2001) The emergence and dissemination of whitefly-transmitted geminiviruses in Latin America. Arch Virol 146:415–441

    Article  PubMed  CAS  Google Scholar 

  4. Mansoor S, Briddon RW, Bull SE, Bedford ID, Bashir A, Hussain M, Saeed M, Zafar Y, Malik KA, Fauquet C, Markham PG (2003) Cotton leaf curl disease is associated with multiple monopartite begomoviruses supported by single DNA beta. Arch Virol 148:1969–1986

    Article  PubMed  CAS  Google Scholar 

  5. Hamilton WD, Stein VE, Coutts RH, Buck KW (1984) Complete nucleotide sequence of the infectious cloned DNA components of tomato golden mosaic virus: potential coding regions and regulatory sequences. EMBO J 3:2197–2205

    PubMed  CAS  Google Scholar 

  6. Fontes EPB, Gladfelter HJ, Schaffer RL, Petty ITD, Hanley-Bowdoin L (1994) Geminivurus replication origins have a modular organization. The Plant Cell 3:405–416

    Google Scholar 

  7. Hanley-Bowdoin L, Settlage SB, Robertson D (2004) Reprogramming plant gene expression: a prerequisite to geminivirus DNA replication. Mol Plant Pathol 5:149–156

    Article  PubMed  CAS  Google Scholar 

  8. Settlage SB, See RG, Hanley-Bowdoin L (2005) Geminivirus C3 protein: replication enhancement and protein interactions. J Virol 79:9885–9895

    Article  PubMed  CAS  Google Scholar 

  9. Sunter G, Bisaro DM (1992) Transactivation of geminivirus AR1 and BR1 gene expression by the viral AL2 gene product occurs at the level of transcription. Plant Cell 4:1321–1331

    PubMed  CAS  Google Scholar 

  10. Pooma W, Petty IT (1996) Tomato golden mosaic virus open reading frame AL4 is genetically distinct from its C4 analogue in monopartite geminiviruses. J Gen Virol 77(Pt 8):1947–1951

    Article  PubMed  CAS  Google Scholar 

  11. Dogra SC, Eini O, Rezaian MA, Randles JW (2009) A novel shaggy-like kinase interacts with the Tomato leaf curl virus pathogenicity determinant C4 protein. Plant Mol Biol 71:25–38

    Article  PubMed  CAS  Google Scholar 

  12. Fontenelle MR, Luz DF, Gomes AP, Florentino LH, Zerbini FM, Fontes EP (2007) Functional analysis of the naturally recombinant DNA-A of the bipartite begomovirus Tomato chlorotic mottle virus. Virus Res 126:262–267

    Article  PubMed  CAS  Google Scholar 

  13. Brough CL, Sunter G, Gardiner WE, Bisaro DM (1992) Kinetics of tomato golden mosaic virus DNA replication and coat protein promoter activity in Nicotiana tabacum protoplasts. Virology 187:1–9

    Article  PubMed  CAS  Google Scholar 

  14. Jeffrey JL, Pooma W, Petty IT (1996) Genetic requirements for local and systemic movement of tomato golden mosaic virus in infected plants. Virology 223:208–218

    Article  PubMed  CAS  Google Scholar 

  15. Etessami P, Callis R, Ellwood S, Stanley J (1988) Delimitation of essential genes of cassava latent virus DNA 2. Nucleic Acids Res 16:4811–4829

    Article  PubMed  CAS  Google Scholar 

  16. Sudarshana MR, Berger PH (1998) Nucleotide sequence of both genomic RNAs of a North American tobacco rattle virus isolate. Arch Virol 143:1535–1544

    Article  PubMed  CAS  Google Scholar 

  17. Programa para el Aumento de la Produccion Producción Nacional de Tomate (PACIT). Informe de Progresos 2010-2011. Estadísticas de Producción Nacional, pp 1–13. http://www.inta.gov.ar/laconsulta/. ISSN 1853-6972

  18. Ribeiro SG, de Ávila AC, Bezerra IC, Fernandes JJ, Faria JC, Lima MF, Gilbertson RL, Maciel-Zambolim E, Zerbini FM (1998) Widespread Occurrence of Tomato Geminiviruses in Brazil, Associated with the New Biotype of the Whitefly Vector. Plant Dis 82:830

    Article  Google Scholar 

  19. Viscarret MM, Torres-Jerez I, Agostini de Manero E, López SN, Botto EE, Brown JK (2003) Mitochondrial DNA evidence for a distinct New World group of Bemisia tabaci (Gennadius) (Hemiptera:Aleyrodiadae) indigenous to Argentina and Bolivia, and presence of the Old World B biotype in Argentina A. Ann Entomol Soc Am 96(1):65–72

    Article  CAS  Google Scholar 

  20. Fernandes FR, de Albuquerque LC, de Britto Giordano L, Boiteux LS, de Avila AC, Inoue-Nagata AK (2008) Diversity and prevalence of Brazilian bipartite begomovirus species associated to tomatoes. Virus Genes 36:251–258

    Article  PubMed  CAS  Google Scholar 

  21. Ribeiro SG, Ambrozevicius LP, Avila AC, Bezerra IC, Calegario RF, Fernandes JJ, Lima MF, de Mello RN, Rocha H, Zerbini FM (2003) Distribution and genetic diversity of tomato-infecting begomoviruses in Brazil. Arch Virol 148:281–295

    Article  PubMed  CAS  Google Scholar 

  22. Fauquet CM, Briddon RW, Brown JK, Moriones E, Stanley J, Zerbini M, Zhou X (2008) Geminivirus strain demarcation and nomenclature. Arch Virol 153:783–821

    Article  PubMed  CAS  Google Scholar 

  23. Vaghi Medina CG, López Lambertini PM (2010) Genetic diversity of begomovirus infecting tomato in Argentina. In: 6th international geminivirus symposium. 4th international ssDNA comparative virology workshop. Guanajuato, Mexico

  24. Rodríguez-Pardina PE, Hanada K, Laguna IG, Zerbini FM, Ducasse DA (2011) Molecular characterisation and relative incidence of bean- and soybean-infecting begomoviruses in northwestern Argentina. Ann Appl Biol 158:69–78

    Article  Google Scholar 

  25. Giachero MS, Bernis ME, López Lambertini PM (2007) Mixed infections with begomovirus is widespread in tomato crops in Argentina. In: Abstract in 5th International geminivirus symposium. 3rd international ssDNA comparative virology workshop, Ouro Preto, Brazil, p 65

  26. Davino S, Napoli C, Dellacroce C, Miozzi L, Noris E, Davino M, Accotto GP (2009) Two new natural begomovirus recombinants associated with the tomato yellow leaf curl disease co-exist with parental viruses in tomato epidemics in Italy. Virus Res 143:15–23

    Article  PubMed  CAS  Google Scholar 

  27. Rojas MR, Gilbertson RL, Maxwell DP (1993) Use of degenerate primers in the polymerase chain reaction to detect whitefly-transmitted geminiviruses. Plant Dis 77:340–347

    Article  CAS  Google Scholar 

  28. Inoue-Nagata AK, Albuquerque LC, Rocha WB, Nagata T (2004) A simple method for cloning the complete begomovirus genome using the bacteriophage phi29 DNA polymerase. J Virol Methods 116:209–211

    Article  PubMed  CAS  Google Scholar 

  29. Arguello-Astorga GR, Ruiz-Medrano R (2001) An iteron-related domain is associated to Motif 1 in the replication proteins of geminiviruses: identification of potential interacting amino acid-base pairs by a comparative approach. Arch Virol 146:1465–1485

    Article  PubMed  CAS  Google Scholar 

  30. Albuquerque LC, Martin DP, Avila AC, Inoue-Nagata AK (2010) Characterization of tomato yellow vein streak virus, a begomovirus from Brazil. Virus Genes 40:140–147

    Article  PubMed  CAS  Google Scholar 

  31. Fernandes JJ, Carvalho MG, Andrade EC, Brommonschenkel SH, Fontes EP, Zerbini FM (2006) Biological and molecular properties of Tomato rugose mosaic virus (ToRMV), a new tomato-infecting begomovirus from Brazil. Plant Pathol 55:513–522

    Article  CAS  Google Scholar 

  32. Katoh K, Misawa K, Kuma K, Miyata T (2002) MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Res 30:3059–3066

    Article  PubMed  CAS  Google Scholar 

  33. Katoh K, Kuma K, Toh H, Miyata T (2005) MAFFT version 5: improvement in accuracy of multiple sequence alignment. Nucleic Acids Res 33:511–518

    Article  PubMed  CAS  Google Scholar 

  34. Schmidt HA, Strimmer K, Vingron M, von Haeseler A (2002) TREE-PUZZLE: maximum likelihood phylogenetic analysis using quartets and parallel computing. Bioinformatics 18:502–504

    Article  PubMed  CAS  Google Scholar 

  35. Strimmer K, von Haeseler A (1997) Likelihood-mapping: a simple method to visualize phylogenetic content of a sequence alignment. Proc Natl Acad Sci USA 94:6815–6819

    Article  PubMed  CAS  Google Scholar 

  36. Guindon S, Dufayard JF, Lefort V, Anisimova M, Hordijk W, Gascuel O (2010) New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Syst Biol 59:307–321

    Article  PubMed  CAS  Google Scholar 

  37. Wyant PS, Gotthardt D, Schafer B, Krenz B, Jeske H (2011) The genomes of four novel begomoviruses and a new Sida micrantha mosaic virus strain from Bolivian weeds. Arch Virol 156:347–352

    Article  PubMed  CAS  Google Scholar 

  38. Guenoune-Gelbart D, Sufrin-Ringwald T, Capobianco H, Gaba V, Polston JE, Lapidot M (2010) Inoculation of plants with begomoviruses by particle bombardment without cloning: Using rolling circle amplification of total DNA from infected plants and whiteflies. J Virol Methods 168:87–93

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by AEBIO2444-INTA-2006 and AEBIO-242411-INTA-2009 projects. We thank Ing. Agr. Joaquin Fernández De Ullivarri for providing us with the plant material from Salta isolated for this study.

Author information

Authors and Affiliations

  1. Instituto de Patología Vegetal (IPAVE) CIAP-INTA, Córdoba, Argentina

    C. G. Vaghi Medina & P. M. López Lambertini

Authors
  1. C. G. Vaghi Medina
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. M. López Lambertini
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to P. M. López Lambertini.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (XLS 33 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Vaghi Medina, C.G., López Lambertini, P.M. Tomato dwarf leaf virus, a New World begomovirus infecting tomato in Argentina. Arch Virol 157, 1975–1980 (2012). https://doi.org/10.1007/s00705-012-1355-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-012-1355-2

Keywords

Search

Navigation