Sequencing and analysis of the complete genome of Rana grylio virus (RGV)
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Infection with Rana grylio virus (RGV), an iridovirus isolated in China in 1995, resulted in a high mortality rate in frogs. The complete genome sequence of RGV was determined and analyzed. The genomic DNA was 105,791 bp long, with 106 open reading frames (ORFs). Dot plot analysis showed that the gene order of RGV shared colinearity with three completely sequenced ranaviruses. A phylogenetic tree was constructed based on concatenated sequences of iridovirus 26 core-gene-encoded proteins, and the result showed high bootstrap support for RGV being a member of the genus Ranavirus and that iridoviruses of other genera also clustered closely. A microRNA (miRNA) prediction revealed that RGV could encode 18 mature miRNAs, many of which were located near genes associated with virus replication. Thirty-three repeated sequences were found in the RGV genome. These results provide insight into the genetic nature of RGV and are useful for laboratory diagnosis for vertebrate iridoviruses.
KeywordsRock Bream Rana Grylio Virus Lymphocystis Disease Virus Tiger Frog Virus miRNA Code
This work was funded by National Major Basic Research Program (2010CB126303, 2009CB118704), Knowledge Innovation Program of the Chinese Academy of Sciences (KSCX2-EW-Z-3), National Natural Science Foundation of China (31072239), and an FEBL research grant (Y15B121F01).
Conflict of interest
The authors declare that they have no conflict of interest.
- 1.Boyer M, Yutin N, Pagnier I, Barrassi L, Fournous G, Espinosa L, Robert C, Azza S, Sun S, Rossmann MG, Suzan-Monti M, La Scola B, Koonin EV, Raoult D (2009) Giant Marseillevirus highlights the role of amoebae as a melting pot in emergence of chimeric microorganisms. Proc Natl Acad Sci USA 106(51):21848–21853PubMedCrossRefGoogle Scholar
- 4.Jancovich JK, Chinchar VG, Hyatt A, Miyazaki T, Williams T, Zhang QY (2011) Family Iridoviridae virus taxonomy. In: 9th report of the ICTV. Elsevier, San Diego, pp 193–210Google Scholar
- 14.Zhang QY, Li ZQ, Jiang YL, Liang SC, Gui JF (1996) Preliminary studies on virus isolation and cell infection from disease frog Rana grylio. Acta Hydrobioliogica Sinica 20:390–392 (Chinese with English Abstract)Google Scholar
- 33.Ronquist F, Huelsenbeck J, Teslenko M (2011) Draft MrBayes version 3.2 Manual: tutorials and model summaries. MrBayes: Bayesian Inference of Phylogeny. http://mrbayes.sourceforge.net/manual.php. Accessed November 15, 2011