Powdery mildew is one of the most important cereal diseases worldwide. Genetic analysis has revealed that mutant alleles of the Mlo gene cause broad-spectrum resistance against this pathogen in barley. In this study, the possibility of inducing broad-spectrum powdery mildew resistance against this pathogen by RNAi of the barley Mlo ortholog in wheat was examined using virus-induced gene silencing (VIGS). A clear correlation was found between resistance and accumulation of Mlo-specific siRNAs, raising the possibility of designing powdery mildew resistance in wheat by RNA silencing using both transgenic and non-transgenic approaches.
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We thank Christophe Lacomme for providing the BSMV vector. V.E. is the recipient of a Bolyai Janos Felowship. This work was supported by GAK TRIPATOL.
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The authors declare that they have no competing interests.
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Várallyay, É., Giczey, G. & Burgyán, J. Virus-induced gene silencing of Mlo genes induces powdery mildew resistance in Triticum aestivum . Arch Virol 157, 1345–1350 (2012). https://doi.org/10.1007/s00705-012-1286-y
- Powdery Mildew
- Powdery Mildew Resistance
- Barley Stripe Mosaic Virus
- Systemic Leaf
- Secondary Hypha