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Sequence and phylogenetic data indicate that an orthobunyavirus recently detected in the Yucatan Peninsula of Mexico is a novel reassortant of Potosi and Cache Valley viruses

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Abstract

We determined the complete nucleotide sequences of the small (S) and medium (M) RNA segments of an orthobunyavirus isolated from mosquitoes in the Yucatan Peninsula of Mexico. A 528-nt region of the large (L) RNA segment was also sequenced. The S RNA segment has greatest nucleotide identity to the homologous region of Cache Valley virus (CVV; 98%) followed by Potosi virus (POTV; 89%) and Northway virus (86%). The M RNA segment has 96% nucleotide identity to the homologous region of POTV, and less than 74% nucleotide identity to the homologous regions of all other orthobunyaviruses for which M segment sequence data are available. The L RNA segment has greatest nucleotide identity to the homologous region of POTV (98%) followed by CVV (82%) and Tensaw virus (77%). These data indicate that the virus, tentatively named Cholul virus (CHLV), is a novel reassortant that acquired its S RNA segment from CVV and its M and L RNA segments from POTV. Phylogenetic data support this conclusion.

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Acknowledgments

The authors thank Thomas Briese and Charles Calisher for their support and advice. This study was supported by intramural grants provided by the Center for Integrated Animal Genomics and the Healthy Livestock Initiative at Iowa State University.

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Authors and Affiliations

  1. Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, 2116 Veterinary Medicine, Ames, IA, 50011, USA

    Bradley J. Blitvich & Rungrat Saiyasombat

  2. Departments of Statistics and Genetics, Development and Cell Biology, College of Liberal Arts and Sciences, College Agriculture and Life Sciences, Iowa State University, Ames, IA, USA

    Karin S. Dorman

  3. Laboratorio de Arbovirologia, Universidad Autonoma de Yucatan, Merida, Yucatan, Mexico

    Julian E. Garcia-Rejon, Jose A. Farfan-Ale & Maria A. Loroño-Pino

Authors
  1. Bradley J. Blitvich
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  2. Rungrat Saiyasombat
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  3. Karin S. Dorman
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  4. Julian E. Garcia-Rejon
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  5. Jose A. Farfan-Ale
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  6. Maria A. Loroño-Pino
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Correspondence to Bradley J. Blitvich.

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Blitvich, B.J., Saiyasombat, R., Dorman, K.S. et al. Sequence and phylogenetic data indicate that an orthobunyavirus recently detected in the Yucatan Peninsula of Mexico is a novel reassortant of Potosi and Cache Valley viruses. Arch Virol 157, 1199–1204 (2012). https://doi.org/10.1007/s00705-012-1279-x

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  • DOI: https://doi.org/10.1007/s00705-012-1279-x

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