Archives of Virology

, Volume 157, Issue 6, pp 1177–1182 | Cite as

VP6-sequence-based cutoff values as a criterion for rotavirus species demarcation

  • Jelle Matthijnssens
  • Peter H. Otto
  • Max Ciarlet
  • Ulrich Desselberger
  • Marc Van Ranst
  • Reimar Johne
Brief Report


Indirect immunofluorescence techniques targeting the rotavirus (RV) protein VP6 are used to differentiate RV species. The ICTV recognizes RV species A to E and two tentative species, F and G. A potential new RV species, ADRV-N, has been described. Phylogenetic trees and pairwise identity frequency graphs were constructed with more than 400 available VP6 sequences and seven newly determined VP6 sequences of RVD strains. All RV species were separated into distinct phylogenetic clusters. An amino acid sequence cutoff value of 53% firmly permitted differentiation of RV species, and ADRV-N was tentatively assigned to a novel RV species H (RVH).


Rotavirus Classification Groups Species VP6 



J.M. is supported by an FWO (‘Fonds voor Wetenschappelijk Onderzoek’) postdoctoral fellowship. R.J. is supported by a grant from the Deutsche Forschungsgemeinschaft (JO369/4-1). We are grateful to Muzahed Uddin Ahmed, Josef Bachmeier, Ulrich Löhren, Christian Prins, and Gert-Jan Zuidam for providing clinical samples from chickens.

Supplementary material

705_2012_1273_MOESM1_ESM.pdf (67 kb)
Supplementary material 1 (PDF 66 kb)


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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Jelle Matthijnssens
    • 1
  • Peter H. Otto
    • 2
  • Max Ciarlet
    • 3
  • Ulrich Desselberger
    • 4
  • Marc Van Ranst
    • 1
  • Reimar Johne
    • 5
  1. 1.Laboratory of Clinical and Epidemiological Virology, Department of Microbiology and Immunology, Rega Institute for Medical ResearchUniversity of LeuvenLeuvenBelgium
  2. 2.Federal Research Institute for Animal Health, Institute for Bacterial Infections and ZoonosesFriedrich-Loeffler-InstituteJenaGermany
  3. 3.Clinical Research and DevelopmentNovartis Vaccines and Diagnostics, IncCambridgeUSA
  4. 4.Department of Medicine, Addenbrooke’s HospitalUniversity of CambridgeCambridgeUK
  5. 5.Federal Institute for Risk AssessmentBerlinGermany

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