Skip to main content
SpringerLink
Log in

Detection, prevalence and analysis of emerging porcine parvovirus infections

  • Original Article
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

A number of newly identified porcine parvoviruses had been described during the last decade, but the presence and prevalence of these viruses are unknown in Hungary and only partly known for Europe. The present study was conducted to detect and measure the prevalence of these viruses, namely porcine parvovirus (PPV) 2, PPV3, PPV4, porcine bocavirus (PBoV) 1, PBoV2, PBo-likeV and the 6V and 7V parvoviruses. The prevalence of PPV1 and porcine circovirus type 2 (PCV2) was also investigated. Faecal samples, blood serum samples, organ tissues, foetuses and semen were collected from different swine herds in Hungary and tested by polymerase chain reaction methods specific for the different viruses. The results indicated that all of the examined parvoviruses were present in Hungary, hence in Europe. The prevalence was 18.1% for PCV2, 0.5 % for PPV1, 6.4% for PPV2, 9.7% for PPV3, 6.4% for PPV4, 1.5% for PBo-likeV, 4.8% for PBoV1 and PBoV2 and 1.8% for 6V and 7V. Based on the analysis of partial PPV4 and PBo-likeV sequences, these viruses showed a high degree of sequence conservation, whereas PPV3 and the majority of PPV2, PBoV1, PBoV2, 6V and 7V sequences showed higher variability. Possible sites of recombination were also identified between PBoV1 and PBoV2 genomes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Abinanti FR, Warfield MS (1961) Recovery of a hemadsorbing virus (HADEN) from the gastrointestinal tract of calves. Virology 14:288–289

    Article  PubMed  CAS  Google Scholar 

  2. Adlhoch C, Kaiser M, Ellerbrok H, Pauli G (2010) High prevalence of porcine Hokovirus in German wild boar populations. Virol J 25(7):171. doi:10.1186/1743-422X-7-171

    Article  Google Scholar 

  3. Allander T, Tammi MT, Eriksson M, Bjerkner A, Tiveljung-Lindell A, Andersson B (2005) Cloning of a human parvovirus by molecular screening of respiratory tract samples. Proc Natl Acad Sci USA 102:12891–12896

    Article  PubMed  CAS  Google Scholar 

  4. Arthur JL, Higgins GD, Davidson GP, Givney RC, Ratcliff RM (2009) A novel bocavirus associated with acute gastroenteritis in Australian children. PLoS Pathog 5:e1000391. doi:10.1371/journal.ppat.1000391

    Article  PubMed  Google Scholar 

  5. Binn LN, Lazar EC, Eddy GA, Kajima M (1970) Recovery and characterization of a minute virus of canines. Infect Immun 1:503–508

    PubMed  CAS  Google Scholar 

  6. Blomström AL, Belák S, Fossum C, McKillen J, Allan G, Wallgren P, Berg M (2009) Detection of a novel porcine boca-like virus in the background of porcine circovirus type 2 induced postweaning multisystemic wasting syndrome. Virus Res 146:125–129

    Article  PubMed  Google Scholar 

  7. Blomström AL, Belák S, Fossum C, Fuxler L, Wallgren P, Berg M (2010) Studies of porcine circovirus type 2, porcine boca-like virus and torque teno virus indicate the presence of multiple viral infections in postweaning multisystemic wasting syndrome pigs. Virus Res 152(1–2):59–64

    Article  PubMed  Google Scholar 

  8. Cadar D, Cságola A, Lőrincz M, Tombácz K, Kiss T, Spinu M, Tuboly T (2011) Genetic detection and analysis of porcine bocavirus type 1 (PoBoV1) in European wild boar (Sus scrofa). Virus Genes. doi:10.1007/s11262-011-0650-4

    PubMed  Google Scholar 

  9. Cadar D, Cságola A, Lőrincz M, Tombácz K, Spinu M, Tuboly T (2011) Distribution and genetic diversity of porcine Hokovirus in wild boars. Arch Virol 156:2233–2239

    Google Scholar 

  10. Cheng WX, Jin Y, Duan ZJ, Xu ZQ, Qi HM, Zhang Q, Yu JM, Zhu L, Jin M, Liu N, Cui SX, Li HY, Fang ZY (2008) Human bocavirus in children hospitalized for acute gastroenteritis: a case-control study. Clin Infect Dis 47:161–167

    Article  PubMed  Google Scholar 

  11. Cheng WX, Li JS, Huang CP, Yao DP, Liu N, Cui SX, Jin Y, Duan ZJ (2010) Identification and nearly full-length genome characterization of novel porcine bocaviruses. PLoS One 5(10):e13583. doi:10.1371/journal.pone.0013583

    Article  PubMed  Google Scholar 

  12. Cheung AK, Wu G, Wang D, Bayles DO, Lager KM, Vincent AL (2010) Identification and molecular cloning of a novel porcine parvovirus. Arch Virol 155(5):801–806

    Article  PubMed  CAS  Google Scholar 

  13. Cságola A, Kecskeméti S, Kardos G, Kiss I, Tuboly T (2006) Genetic characterization of type 2 porcine circoviruses detected in Hungarian wild boars. Arch Virol 151(3):495–507

    Article  PubMed  Google Scholar 

  14. Cságola A, Lőrincz M, Tombácz K, Biksi I, Balka GY, Tuboly T (2009) Detection of human parvovirus 4 related porcine hokoviruses in Hungary. Acta Microbiol Immunol Hung 56:135

    Google Scholar 

  15. Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysisprogram for Windows 95/98/NT. Nucl Acids Symp Ser 41:95–98

    CAS  Google Scholar 

  16. Hijikata M, Abe K, Win KM, Shimizu YK, Keicho N, Yoshikura H (2001) Identification of new parvovirus DNA sequence in swine sera from Myanmar. Jpn J Infect Dis 54:244–245

    PubMed  CAS  Google Scholar 

  17. Huang L, Zhai SL, Cheung AK, Zhang HB, Long JX, Yuan SS (2010) Detection of a novel porcine parvovirus, PPV4, in Chinese swine herds. Virol J 21(7):333. doi:10.1186/1743-422X-7-333

    Article  Google Scholar 

  18. Lau SK, Woo PC, Tse H, Fu CT, Au WK, Chen XC, Tsoi HW, Tsang TH, Chan JS, Tsang DN, Li KS, Tse CW, Ng TK, Tsang OT, Zheng BJ, Tam S, Chan KH, Zhou B, Yuen KY (2008) Identification of novel porcine and bovine parvoviruses closely related to human parvovirus 4. J Gen Virol 89:1840–1848

    Article  PubMed  CAS  Google Scholar 

  19. Kapoor A, Slikas E, Simmonds P, Chieochansin T, Naeem A, Shaukat S, Alam MM, Sharif S, Angez M, Zaidi S, Delwart E (2009) A newly identified bocavirus species in human stool. J Infect Dis 199:196–200

    Article  PubMed  Google Scholar 

  20. McKillen J, McNeilly F, Duffy C, McMenamy M, McNair I, Hjertner B, Millar A, McKay K, Lagan P, Adair B, Allan G (2011) Isolation in cell cultures and initial characterisation of two novel bocavirus species from swine in Northern Ireland. Vet Microbiol. doi:10.1016/j.vetmic.2011.04.013 (in press)

  21. Shan T, Lan D, Li L, Wang C, Cui L, Zhang W, Hua X, Zhu C, Zhao W, Delwart E (2011) Genomic characterization and high prevalence of bocaviruses in swine. PLoS One 6(4):e17292. doi:10.1371/journal.pone.0017292

    Article  PubMed  CAS  Google Scholar 

  22. Soares RM, Durigon EL, Bersano JG, Richtzenhain LJ (1999) Detection of porcine parvovirus DNA by the polymerase chain reaction assay using primers to the highly conserved nonstructural protein gene, NS-1. J Virol Methods 78(1–2):191–198

    Article  PubMed  CAS  Google Scholar 

  23. Szelei J, Liu K, Li Y, Fernandes S, Tijssen P (2010) Parvovirus 4-like virus in blood products. Emerg Infect Dis 16(3):561–564

    Article  PubMed  CAS  Google Scholar 

  24. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. doi:10.1093/molbev/msr121

    Google Scholar 

  25. Tozer SJ, Lambert SB, Whiley DM, Bialasiewicz S, Lyon MJ, Nissen MD, Sloots TP (2009) Detection of human bocavirus in respiratory, fecal, and blood samples by real-time PCR. J Med Virol 81:488–493

    Article  PubMed  CAS  Google Scholar 

  26. Wang F, Wei Y, Zhu C, Huang X, Xu Y, Yu L, Yu X (2010) Novel parvovirus sublineage in the family of Parvoviridae. Virus Genes 41:305–308

    Article  PubMed  CAS  Google Scholar 

  27. Zhai S, Yue C, Wei Z, Long J, Ran D, Lin T, Deng Y, Huang L, Sun L, Zheng H, Gao F, Zheng H, Chen S, Yuan S (2010) High prevalence of a novel porcine bocavirus in weanling piglets with respiratory tract symptoms in China. Arch Virol 155(8):1313–1317

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This paper was supported by the János Bolyai Research Scholarship of the Hungarian Academy of Science, grant NKB15945 from the Szent István University and OTKA research grant PD100405.

Author information

Authors and Affiliations

  1. Faculty of Veterinary Science, Department of Microbiology and Infectious Diseases, Szent István University, Hungária krt. 23–25, 1143, Budapest, Hungary

    Attila Cságola, Márta Lőrincz, Kata Tombácz & Tamás Tuboly

  2. Faculty of Veterinary Science, Large Animals Clinic, Szent István University, Dóra major, 2225, Üllő, Hungary

    Imre Biksi

  3. Department of Infectious Diseases, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, Manastur 3-5, 400372, Cluj-Napoca, Romania

    Dániel Cadar

Authors
  1. Attila Cságola
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Márta Lőrincz
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Dániel Cadar
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kata Tombácz
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Imre Biksi
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Tamás Tuboly
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Tamás Tuboly.

Additional information

Nucleotide sequence data reported here are available in the GenBank database under the following accession numbers: JN400849 (PBo-likeV), JN400850-N400861 (PPV2), JN400862-JN400868 (PPV3), JN400869 (PPV4), JN400870-JN400875 (PBoV), JN400876-JN400879 (6V7V).

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cságola, A., Lőrincz, M., Cadar, D. et al. Detection, prevalence and analysis of emerging porcine parvovirus infections. Arch Virol 157, 1003–1010 (2012). https://doi.org/10.1007/s00705-012-1257-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-012-1257-3

Keywords

Search

Navigation