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Characterization of Epstein-Barr virus type 1 nuclear antigen 3C sequence patterns of nasopharyngeal and gastric carcinomas in northern China

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Abstract

Epstein-Barr virus nuclear antigen protein 3C (EBNA3C) is a 992-amino-acid protein that has been shown to play a complex regulatory role in the transcription of viral and cellular genes. In this study, we successfully amplified 26 Epstein-Barr virus (EBV)-associated gastric carcinomas (EBVaGCs), 50 nasopharyngeal carcinomas (NPCs) and 27 throat washing (TW) samples from healthy donors. Based on a phylogenetic tree, the samples could be divided into three patterns. 3C-6 was the predominant subtype in northern China, and the variations between the strains sequenced in our study and those from southern China and Japan were similar, but differences were also identified. The distribution of EBNA3C subtypes among EBVaGCs, NPCs and healthy donors was not significantly different. These data suggest that EBNA3C gene variations are geographically restricted rather than tumor-specific polymorphisms.

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Acknowledgments

This work was supported by the grant from National Natural Science Foundation of China (NSFC 30740068), the Natural Science Foundation of Shandong Province (Y2008C90) and Science and Technology of Qingdao City (08-2-3-7-hz).

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Authors and Affiliations

  1. Department of Medical Microbiology, Qingdao University Medical College, 38 Dengzhou Road, Qingdao, 266021, China

    Guocai Wu, Yun Wang & Bing Luo

  2. Department of Clinical Laboratory, Guangdong Provincial Hospital of Chinese Medicine, 111 Dade Road, Guangzhou, 510120, China

    Yan Chao

  3. Department of Clinical Laboratory, Weifang People’s Hospital, 151 Guangwen Road, Weifang, 261000, China

    Yuping Jia

  4. Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, USA

    Chengquan Zhao

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  1. Guocai Wu
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  2. Yun Wang
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Correspondence to Bing Luo.

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Wu, G., Wang, Y., Chao, Y. et al. Characterization of Epstein-Barr virus type 1 nuclear antigen 3C sequence patterns of nasopharyngeal and gastric carcinomas in northern China. Arch Virol 157, 845–853 (2012). https://doi.org/10.1007/s00705-012-1241-y

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