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The serogroup A capsular polysaccharide from Neisseria meningitidis enhances the cell-mediated immunity and the protective capacity induced by a dengue fusion protein in mice

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Abstract

We previously tested in monkeys the P64k-DomIII fusion protein of DEN-2 (PD5), combined with the serogroup A capsular polysaccharide (CPS-A) from N. meningitidis as an immunopotentiator. The results revealed the induction of neutralizing antibodies and partial protection after DEN-2 challenge. Since one formulation of the CPS-A was only evaluated in monkeys, in the present study, we evaluated two CPS-A-based formulations in mice. Animals immunized with PD5 in alum with the highest dose of CPS-A produced the highest levels of INF-γ secretion upon viral stimulation, and accordingly, 100% protection. This is the first report that describes the dose effect of CPS-A and its capacity to potentiate the cell-mediated immunity induced by a heterologous antigen in mice.

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Acknowledgments

The authors are very grateful to Dr. Ricardo Silva (CIGB) and Dr. Harold Curiel (Center of Biomolecular Chemistry) for their critical reading of the manuscript. The authors wish to thank to colleagues from the Conjugate-Vaccine Department for assistance in the preparations of formulations.

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Authors and Affiliations

  1. Vaccine Division, Center for Genetic Engineering and Biotechnology (CIGB), Avenue 31, P.O. Box 6162, Havana 6, 10 600, Cuba

    Lisset Hermida, Iris Valdés, Lázaro Gil, Laura Lazo, Yaremis Romero & Gerardo Guillén

  2. Department of Virology, PAHO/WHO Collaborating Center for the study of Dengue and its vector, ‘‘Pedro Kourí’’ Tropical Medicine Institute (IPK), P.O. Box. 601, Havana, Cuba

    Lídice Bernardo & María G. Guzmán

Authors
  1. Lisset Hermida
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  2. Iris Valdés
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  3. Lázaro Gil
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  4. Lídice Bernardo
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  5. Laura Lazo
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  6. Yaremis Romero
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  7. María G. Guzmán
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  8. Gerardo Guillén
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Correspondence to Lisset Hermida.

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Hermida, L., Valdés, I., Gil, L. et al. The serogroup A capsular polysaccharide from Neisseria meningitidis enhances the cell-mediated immunity and the protective capacity induced by a dengue fusion protein in mice. Arch Virol 157, 987–991 (2012). https://doi.org/10.1007/s00705-012-1239-5

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  • DOI: https://doi.org/10.1007/s00705-012-1239-5

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