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Differentiation of infectious bursal disease virus (IBDV) genome segment B of very virulent and classical lineage by RT-PCR amplification and restriction enzyme analysis

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Abstract

Molecular characterization of IBDV usually relies on the analysis of segment A of the bi-segmented, double-stranded RNA genome. Although segments B of classical and very virulent IBDVs differ significantly, re-assortment of genome segments does occur, and molecular epidemiological studies demand the analysis of both segments. An RT-PCR and restriction enzyme analysis for molecular discrimination between genome segment B of classical and very virulent IBDVs is described. Tested on eight IBDV strains/isolates, the protocol successfully identified very virulent and classical IBDVs as well as a segment reassortant. This approach is a valuable tool for molecular epidemiological studies on IBDV.

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Authors and Affiliations

  1. Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh

    M. R. Islam, S. Rahman, M. Noor & E. H. Chowdhury

  2. Faculty of Veterinary Medicine, Institute for Virology, University of Leipzig, 04103, Leipzig, Germany

    H. Müller

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  1. M. R. Islam
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  2. S. Rahman
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  3. M. Noor
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  4. E. H. Chowdhury
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  5. H. Müller
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Correspondence to M. R. Islam.

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Islam, M.R., Rahman, S., Noor, M. et al. Differentiation of infectious bursal disease virus (IBDV) genome segment B of very virulent and classical lineage by RT-PCR amplification and restriction enzyme analysis. Arch Virol 157, 333–336 (2012). https://doi.org/10.1007/s00705-011-1159-9

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  • DOI: https://doi.org/10.1007/s00705-011-1159-9

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