Inhibition of Lassa virus and Ebola virus infection in host cells treated with the kinase inhibitors genistein and tyrphostin

Abstract

Arenaviruses and filoviruses are capable of causing hemorrhagic fever syndrome in humans. Limited therapeutic and/or prophylactic options are available for humans suffering from viral hemorrhagic fever. In this report, we demonstrate that pre-treatment of host cells with the kinase inhibitors genistein and tyrphostin AG1478 leads to inhibition of infection or transduction in cells infected with Ebola virus, Marburg virus, and Lassa virus. In all, the results demonstrate that a kinase inhibitor cocktail consisting of genistein and tyrphostin AG1478 is a broad-spectrum antiviral that may be used as a therapeutic or prophylactic against arenavirus and filovirus hemorrhagic fever.

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Acknowledgments

The authors would like to acknowledge Drs. James Blank, Herbert Bresler, and Catherine Smith for providing their support and technical assistance. This work was funded through the Battelle Health and Life Sciences Independent Research and Development Program.

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Correspondence to Eric M. Vela.

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Kolokoltsov, A.A., Adhikary, S., Garver, J. et al. Inhibition of Lassa virus and Ebola virus infection in host cells treated with the kinase inhibitors genistein and tyrphostin. Arch Virol 157, 121–127 (2012). https://doi.org/10.1007/s00705-011-1115-8

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Keywords

  • Arenavirus
  • Filovirus
  • Lassa virus
  • Ebola virus
  • Kinase inhibitors