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Hepatitis B virus X protein enhances activation of nuclear factor κB through interaction with valosin-containing protein

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Abstract

Hepatitis B virus X protein (HBx protein) is a multifunctional regulatory protein. The transactivation of nuclear factor kappa B (NF-κB) by HBx protein has been shown to be of importance in the pathogenesis of HBV-related diseases. However, the mechanism involved remains largely unclear. In this study, a CytoTrap yeast two-hybrid system was employed to screen binding partners of the HBx protein; 29 cellular proteins, including valosin-containing protein (VCP), were identified. The interaction between HBx protein and VCP was further confirmed in vitro and in vivo using a glutathione S-transferase pull-down assay and co-immunoprecipitation, respectively. It was also shown that this interaction is mediated by amino acid residues 51–120 of the HBx protein. In Huh-7 hepatoma cells, HBx protein enhanced the VCP-mediated activation of NF-κB. Our findings provide new insights into the molecular mechanisms that lead to the activation of NF-κB by HBx protein.

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Acknowledgments

This work was supported by grants from Key Program of Scientific Research of Fujian Medical University (09ZD004), Program for Innovative Research Team in Science and Technology in Fujian Province University (FMU-RT001), the Science Research Foundation of the Ministry of Health and the United Fujian Provincial Health and Education Project for Tackling the Key Research (WKJ2008-2-056). None of the authors has a financial interest in this work.

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Authors and Affiliations

  1. Key Laboratory of Ministry of Education for Gastrointestinal Cancer, Research Center of Molecular Medicine, Fujian Medical University, 88 Jiaotong Road, Fuzhou, 350004, People’s Republic of China

    Bo-Yan Jiao, Wan-Song Lin, Fei-Fei She, Wan-Nan Chen & Xu Lin

  2. Key Laboratory of Tumor Microbiology, Department of Medical Microbiology, Fujian Medical University, Fuzhou City, 350004, People’s Republic of China

    Wan-Nan Chen & Xu Lin

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  1. Bo-Yan Jiao
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  2. Wan-Song Lin
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  3. Fei-Fei She
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  4. Wan-Nan Chen
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  5. Xu Lin
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Correspondence to Xu Lin.

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B.-Y. Jiao and W.-S. Lin are contributed equally in this study.

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Jiao, BY., Lin, WS., She, FF. et al. Hepatitis B virus X protein enhances activation of nuclear factor κB through interaction with valosin-containing protein. Arch Virol 156, 2015–2021 (2011). https://doi.org/10.1007/s00705-011-1099-4

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  • DOI: https://doi.org/10.1007/s00705-011-1099-4

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