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Genome of low-temperature T4-related bacteriophage vB_EcoM-VR7

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Abstract

The complete genome sequence of the T4-related low-temperature Escherichia coli bacteriophage vB_EcoM-VR7 was determined. The genome sequence is 169,285 bp long, with a G+C content of 40.3%. Overall, 95% of the phage genome is coding. It encodes 293 putative protein-encoding open reading frames (ORFs) and tRNAMet. More than half (59%) of the genomic DNA lacks significant homology with the DNA of T4, but once translated, 72% of the vB_EcoM-VR7 genome (211 ORFs) encodes protein homologues of T4 genes. Overall, 46 vB_EcoM-VR7 ORFs have no homologues in T4 but are derived from other T4-related phages, nine ORFs show similarities to bacterial or non-T4-related phage genes, and 27 ORFs are unique to vB_EcoM-VR7. This phage lacks several T4 enzymes involved in host DNA degradation; however, there is extensive representation of the DNA replication, recombination and repair enzymes as well as the viral capsid and tail structural genes.

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References

  1. Balter M (2000) Evolution on life’s fringes. Science 289:1866–1867

    Article  PubMed  CAS  Google Scholar 

  2. Karam JD, Miller ES (2010) Bacteriophage T4 and its relatives. Virol J 7:293. doi:10.1186/1743-422X-7-293

  3. Petrov VM, Ratnayaka S, Nolan JM, Miller ES, Karam JD (2010) Genomes of the T4-related bacteriophages as windows on microbial genome evolution. Virol J 7:292. doi:10.1186/1743-422X-7-292

  4. Comeau AM, Bertrand C, Letarov A, Tétart F, Krisch HM (2007) Modular architecture of the T4 phage superfamily: a conserved core genome and a plastic periphery. Virology 362:384–396. doi:10.1016/j.virol.2006.12.031

    Article  PubMed  CAS  Google Scholar 

  5. Ackermann H-W, Krisch HM (1997) A catalogue of T4-type bacteriophages. Arch Virol 142:2329–2345

    Article  PubMed  CAS  Google Scholar 

  6. Leclerc H, Mossel DAA, Edberg SC, Struijk CB (2001) Advances in the bacteriology of the coliform group: their suitability as markers of microbial water safety. Annu Rev Microbiol 55:201–234

    Article  PubMed  CAS  Google Scholar 

  7. Seeley ND, Primrose SB (1980) The effect of temperature on the ecology of aquatic bacteriophages. J Gen Virol 46:87–95

    Article  Google Scholar 

  8. Kaliniene L, Klausa V, Truncaite L (2010) Low-temperature T4-like coliphages vB_EcoM-VR5, vB_EcoM-VR7 and vB_EcoM-VR20. Arch Virol 155:871–880

    Article  PubMed  CAS  Google Scholar 

  9. Klausa V, Piešinienė L, Staniulis J, Nivinskas R (2003) Abundance of T4-type bacteriophages in municipal wastewater and sewage. Ekologija 1:47–50

    Google Scholar 

  10. Miller ES, Kutter E, Mosig G, Arisaka F, Kunisawa T, Rüger W (2003) Bacteriophage T4 genome. Microbiol Mol Biol Rev 67:86–156

    Article  PubMed  CAS  Google Scholar 

  11. Wilson GW, Edgell DR (2009) Phage T4 mobE promoters trans homing of the defunct homing endonuclease I-TevIII. Nucl Acids Res 21:7110–7123

    Article  Google Scholar 

  12. Edgell DR, Gibb EA and Belfort M (2010) Mobile DNA elements in T4 and related phages: a review in the series on bacteriophage T4 and its relatives. Virol J 7:290. doi:10.1186/1743-422X-7-290

  13. Comeau AM, Krish HM (2008) The capsid of the T4 phage superfamily: the evolution, diversity, and structure of some of the most prevalent proteins in the biosphere. Mol Biol Evol 25:1321–1332

    Article  PubMed  CAS  Google Scholar 

  14. Comeau AM, Bertrand C, Letarov A, Tetart F, Krish HM (2007) Modular architecture of the T4 phage superfamily: a conserved core genome and a plastic periphery. Virology 362:384–396

    Article  PubMed  CAS  Google Scholar 

  15. Chibani-Chennoufi S, Canchaya C, Bruttin A, Brüssow H (2004) Comparative genomics of the T4-like Escherichia coli phage JS98: implications for the evolution of T4 phages. J Bacteriol 186(24):8276–8286. doi:10.1128/JB.186.24.8276-8286.2004

    Google Scholar 

  16. Black LW, Showe MK, Steven AC (1994) Morphogenesis of the T4 head. In: Karam JD (ed) Molecular biology of bacteriophage T4. American Society for Microbiology, Washington, DC, pp 369–382

  17. Petrov VM, Nolan JM, Bertrand C, Levy D, Desplats C, Krish HM, Karam JD (2006) Plasticity of the gene functions for DNA replication in the T4-like phages. J Mol Biol 361:46–68

    Article  PubMed  CAS  Google Scholar 

  18. Filée J, Bapteste E, Susko E, Krisch HM (2006) A selective barrier to horizontal gene transfer in the T4-type bacteriophages that has preserved a core genome with the viral replication and structural genes. Mol Biol Evol 23:1688–1696

    Article  PubMed  Google Scholar 

  19. Nolan JM, Petrov V, Bertrand C, Krish HM, Karam JD (2006) Genetic diversity among five T4-like bacteriophages. Virol J 3:30

    Google Scholar 

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Acknowledgments

We thank Dr. L. W. Black (University of Maryland, Baltimore, US) for E. coli strain BE. This work was funded by the Research Council of Lithuania (grant no. MIP-10240).

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Authors and Affiliations

  1. Vilnius University Institute of Biochemistry, Mokslininkų 12, 08662, Vilnius, Lithuania

    Laura Kaliniene, Vytautas Klausa, Aurelija Zajančkauskaite, Rimas Nivinskas & Lidija Truncaite

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  1. Laura Kaliniene
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  2. Vytautas Klausa
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  3. Aurelija Zajančkauskaite
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  4. Rimas Nivinskas
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  5. Lidija Truncaite
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Correspondence to Laura Kaliniene.

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Kaliniene, L., Klausa, V., Zajančkauskaite, A. et al. Genome of low-temperature T4-related bacteriophage vB_EcoM-VR7. Arch Virol 156, 1913–1916 (2011). https://doi.org/10.1007/s00705-011-1084-y

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  • DOI: https://doi.org/10.1007/s00705-011-1084-y

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