Abstract
Sapporo virus belongs to the genus Sapovirus (family Caliciviridae) and has a non-segmented single-stranded, positive-sense RNA genome. This virus causes acute gastroenteritis in human, porcine and mink hosts. In this study, the complete genome of a Brazilian sapovirus isolate from a child with acute gastroenteritis was determined. A phylogenetic tree was constructed to analyze the genotype of this sapovirus (Sapo_BR-DF01), and possible intra- and inter-genogroups recombination events were evaluated in silico using the RDP3 program. Two inter-genogroup and two intra-genogroup recombination events were newly recognized in this study.
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Acknowledgments
The first author is supported financially by CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior). The fourth and fifth authors are research fellows of CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico). We thank Dr. Tomoichiro Oka (National Institute of Infectious Diseases, Japan) for the technical discussion.
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705_2011_1079_MOESM1_ESM.ppt
Supplementary Fig. 1 Schematic representation of the sapovirus genome and the position of primers used for RT-PCR, showing overlapping regions of the clones. Supplementary material 1 (PPT 115 kb)
705_2011_1079_MOESM2_ESM.ppt
Supplementary Fig. 2 Recombination analysis based on the 3′ region of the sapovirus genome using the RDP method. A schematic representation of the genomic region used in this analysis is shown below, to scale, with the corresponding nucleotide positions. Longer boxes represent the genome, and the predicted recombinant regions are shown as smaller boxes below each genome. The possible parental or daughter sequences are indicated by dotted lines. The genogroup-genotype classification is indicated at the right. p-values identified by RDP for each recombination event are indicated below the virus identifier. Supplementary material 2 (PPT 162 kb)
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dos Anjos, K., Lima, L.M.P., Silva, P.A. et al. The possible molecular evolution of sapoviruses by inter- and intra-genogroup recombination. Arch Virol 156, 1953–1959 (2011). https://doi.org/10.1007/s00705-011-1079-8
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DOI: https://doi.org/10.1007/s00705-011-1079-8